Ravi Sunil K, Narasingappa Ramesh B, Mundagaru Ravi, Girish Talakatta K, Vincent Bruno
Department of Biotechnology, College of Agriculture, University of Agriculture Sciences, Bangalore, Hassan, India.
Pharmacology laboratory, SDM Centre for Research in Ayurveda and Allied Sciences, Kuthpady, Udupi, India.
J Pharm Pharmacol. 2020 Aug;72(8):1119-1132. doi: 10.1111/jphp.13283. Epub 2020 May 3.
To examine the ability of Cassia tora extract to produce, in vitro and in vivo, beneficial effects with respect to events occurring during Alzheimer's disease.
Previously characterised methanol extract of C. tora was tested for its ability to lessen Aβ aggregation processes in vitro and to alleviate aluminium-induced impairments in vivo in rats.
Cassia tora extract prevents the aggregation of monomeric, oligomeric and fibrillary Aβ in vitro. Moreover, the daily ingestion of 100 and 400 milligrams of the extract per kilogram of body weight for 60 days ameliorates the neurobehavioral and cognitive abilities of aluminium-treated rats in vivo. Importantly, treatments with the extract trigger a significant recovery of antioxidant enzymes function, a diminution of lipid peroxidation and acetylcholinesterase activity, a decrease of pro-inflammatory cytokines expression and an increase of brain-derived neurotrophic factor levels in both the hippocampus and the frontal cortex. Finally, we evidence that the extract is able to ameliorate the aluminium-dependent loss of neuronal integrity in the CA1 and CA3 regions of the hippocampus.
Altogether, our results reveal that methanol extract of C. tora is able to prevent typical AD-related events and therefore stands as a promising mild and natural anti-AD multitarget compound.
研究决明子提取物在体外和体内对阿尔茨海默病发生过程中相关事件产生有益作用的能力。
对先前已表征的决明子甲醇提取物进行测试，以考察其在体外减轻Aβ聚集过程以及在体内减轻铝诱导的大鼠损伤的能力。
决明子提取物在体外可防止单体、寡聚体和纤维状Aβ的聚集。此外，每天按每千克体重100毫克和400毫克的剂量摄入该提取物，持续60天，可改善体内铝处理大鼠的神经行为和认知能力。重要的是，用该提取物进行处理可使抗氧化酶功能显著恢复，脂质过氧化和乙酰胆碱酯酶活性降低，促炎细胞因子表达减少，海马体和额叶皮质中脑源性神经营养因子水平升高。最后，我们证明该提取物能够改善海马体CA1和CA3区域中铝依赖性神经元完整性的丧失。
总之，我们的结果表明决明子甲醇提取物能够预防典型的阿尔茨海默病相关事件，因此是一种有前景的温和天然抗阿尔茨海默病多靶点化合物。
