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核输入的 LIKE 异染色质蛋白 1 可被输入蛋白 α-1、α-2 和 α-3 冗余地介导。

Nuclear import of LIKE HETEROCHROMATIN PROTEIN1 is redundantly mediated by importins α-1, α-2 and α-3.

机构信息

Department of Molecular Genetic Biotechnology, Dong-A University, Busan, 604-714, Korea.

Systems and Synthetic Agrobiotech Center, Gyeongsang National University, Jinju, 660-701, Korea.

出版信息

Plant J. 2020 Aug;103(3):1205-1214. doi: 10.1111/tpj.14796. Epub 2020 Jun 1.

DOI:10.1111/tpj.14796
PMID:32365248
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7810169/
Abstract

LIKE HETEROCHROMATIN PROTEIN1 (LHP1) encodes the only plant homologue of the metazoan HETEROCHROMATIN PROTEIN1 (HP1) protein family. The LHP1 protein is necessary for proper epigenetic regulation of a range of developmental processes in plants. LHP1 is a transcriptional repressor of flowering-related genes, such as FLOWERING LOCUS T (FT), FLOWERING LOCUS C (FLC), AGAMOUS (AG) and APETALA 3 (AP3). We found that LHP1 interacts with importin α-1 (IMPα-1), importin α-2 (IMPα-2) and importin α-3 (IMPα-3) both in vitro and in vivo. A genetic approach revealed that triple mutation of impα-1, impα-2 and impα-3 resulted in Arabidopsis plants with a rapid flowering phenotype similar to that of plants with mutations in lhp1 due to the upregulation of FT expression. Nuclear targeting of LHP1 was severely impaired in the impα triple mutant, resulting in the de-repression of LHP1 target genes AG, AP3 and SHATTERPROOF 1 as well as FT. Therefore, the importin proteins IMPα-1, -2 and -3 are necessary for the nuclear import of LHP1.

摘要

LIKE HETEROCHROMATIN PROTEIN1 (LHP1) 编码了真核生物 HETEROCHROMATIN PROTEIN1 (HP1) 蛋白家族中唯一的植物同源物。LHP1 蛋白对于植物中一系列发育过程的正确表观遗传调控是必要的。LHP1 是开花相关基因（如 FLOWERING LOCUS T (FT)、FLOWERING LOCUS C (FLC)、AGAMOUS (AG) 和 APETALA 3 (AP3)）的转录抑制剂。我们发现 LHP1 体外和体内均与输入蛋白 α-1（IMPα-1）、输入蛋白 α-2（IMPα-2）和输入蛋白 α-3（IMPα-3）相互作用。遗传方法表明，impα-1、impα-2 和 impα-3 的三重突变导致拟南芥植物具有快速开花表型，类似于 lhp1 突变植物，因为 FT 表达上调。IMPα 三重突变体中 LHP1 的核靶向严重受损，导致 LHP1 靶基因 AG、AP3 和 SHATTERPROOF 1 以及 FT 的去抑制。因此，输入蛋白 IMPα-1、-2 和 -3 对于 LHP1 的核输入是必要的。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4c8d/7810169/0a39ad3f25b1/nihms-1601237-f0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4c8d/7810169/70f37b1ae6ca/nihms-1601237-f0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4c8d/7810169/db941ca8e23b/nihms-1601237-f0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4c8d/7810169/99037d520e39/nihms-1601237-f0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4c8d/7810169/ecf8c016f044/nihms-1601237-f0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4c8d/7810169/affab0446f22/nihms-1601237-f0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4c8d/7810169/1840fc336ea6/nihms-1601237-f0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4c8d/7810169/0a39ad3f25b1/nihms-1601237-f0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4c8d/7810169/70f37b1ae6ca/nihms-1601237-f0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4c8d/7810169/db941ca8e23b/nihms-1601237-f0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4c8d/7810169/99037d520e39/nihms-1601237-f0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4c8d/7810169/ecf8c016f044/nihms-1601237-f0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4c8d/7810169/affab0446f22/nihms-1601237-f0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4c8d/7810169/1840fc336ea6/nihms-1601237-f0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4c8d/7810169/0a39ad3f25b1/nihms-1601237-f0007.jpg

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