Oral Biology Research Unit, Faculty of Dentistry, Thammasat University (Rangsit Campus), Pathum Thani, Thailand
Oral Biology Research Unit, Faculty of Dentistry, Thammasat University (Rangsit Campus), Pathum Thani, Thailand.
Anticancer Res. 2020 May;40(5):2657-2665. doi: 10.21873/anticanres.14236.
BACKGROUND/AIM: Nitric oxide (NO) functions have been studied in many cancer types, but rarely in head and neck squamous cell carcinoma (HNSCC). This study aimed to investigate the behavior of HNSCC cells following exposure to high NO (HNO).
Two pairs of isogenic HNSCC cell lines (HN18/HN17, HN30/HN31) were used, and were treated with a NO donor for 72 h. Cell viability, cell cycle, apoptosis, invasion, and MMP activity were determined using MTT, flow cytometry, Matrigel invasion, and gelatinase zymography assays, respectively.
HNO induced HN18 and HN31 cell cycle progression in S and G/M phases. Anti-invasion, MMP-2 inhibition, and apoptosis induction were observed in certain HNO-adapted cell lines. High NO did not affect MMP-9 activity in all cell lines.
NO enhanced cell cycle progression and apoptosis but inhibited cell invasion in HNSCC cells.
背景/目的：一氧化氮（NO）的功能已在许多癌症类型中得到研究，但在头颈部鳞状细胞癌（HNSCC）中很少研究。本研究旨在研究 HNSCC 细胞在暴露于高浓度 NO（HNO）后的行为。
使用两对同源 HNSCC 细胞系（HN18/HN17、HN30/HN31），并用一氧化氮供体处理 72 小时。使用 MTT、流式细胞术、Matrigel 侵袭和明胶酶谱分析分别测定细胞活力、细胞周期、细胞凋亡、侵袭和 MMP 活性。
HNO 诱导 HN18 和 HN31 细胞在 S 和 G/M 期的细胞周期进展。在某些适应 HNO 的细胞系中观察到抗侵袭、MMP-2 抑制和细胞凋亡诱导。高浓度的 NO 对所有细胞系的 MMP-9 活性没有影响。
NO 增强了 HNSCC 细胞的细胞周期进程和细胞凋亡，但抑制了细胞侵袭。
