Ma Qinge, Wei Rongrui, Sang Zhipei
Key Laboratory of Modern Preparation of TCM of Ministry of Education, Research Center of Natural Resources of Chinese Medicinal Materials and Ethnic Medicine, Jiangxi University of Traditional Chinese Medicine, Nanchang, China.
College of Chemistry and Pharmaceutical Engineering, Nanyang Normal University, Nanyang, China.
J Food Biochem. 2020 Jul;44(7):e13264. doi: 10.1111/jfbc.13264. Epub 2020 May 4.
Cucumis bisexualis has been widely served as a beneficial wild fruit in China. Four new homoisoflavonoids (1-4), together with eight known homoisoflavonoid derivatives (5-12) were isolated from the 90% EtOH extract of C. bisexualis, using repeated open column chromatography and preparative thin-layer chromatography. They were elucidated based on the analysis of spectral data and references from the fruits of C. bisexualis for the first time. These compounds (1-12) were evaluated for their in vitro inhibitory effects against the increase of aspartate aminotransferase and alanine aminotransferase levels in H O -induced HepG2 cells in the range of their maximum nontoxic concentration. Among them, compounds 3, 4, 8, and 9 exhibited certain hepatoprotective activities. PRACTICAL APPLICATIONS: Cucumis bisexualis A.M. Lu ＆ G.C. Wang is an edible and medical fruit with many functional properties. A detailed study was made to identify the bioactive constituents of C. bisexualis and four new homoisoflavonoids (1-4) and eight known homoisoflavonoid derivatives (5-12) were isolated from the fruits of C. bisexualis for the first time. Compounds 3, 4, 8, and 9 exhibited certain hepatoprotective activities. This study suggests that natural compounds isolated from C. bisexualis could be used as promising hepatoprotective agents.
两性黄瓜在中国一直作为有益的野生水果被广泛食用。采用反复开放柱色谱法和制备薄层色谱法，从两性黄瓜90%乙醇提取物中分离得到4个新的高异黄酮类化合物（1-4）以及8个已知的高异黄酮衍生物（5-12）。首次通过光谱数据分析和参考两性黄瓜果实的文献对它们进行了鉴定。在最大无毒浓度范围内，对这些化合物（1-12）抑制H₂O₂诱导的HepG2细胞中天冬氨酸转氨酶和丙氨酸转氨酶水平升高的体外抑制作用进行了评估。其中，化合物3、4、8和9表现出一定的保肝活性。实际应用：两性黄瓜是一种具有多种功能特性的可食用和药用水果。对两性黄瓜的生物活性成分进行了详细研究，首次从两性黄瓜果实中分离得到4个新的高异黄酮类化合物（1-4）和8个已知的高异黄酮衍生物（5-12）。化合物3、4、8和9表现出一定的保肝活性。该研究表明，从两性黄瓜中分离得到的天然化合物有望作为保肝剂。
