Jia Yu-Chen, Fu Jia-Yue, Huang Ping, Zhang Zhan-Pu, Chao Bo, Bai Jie
Inner Mongolia Key Laboratory of Molecular Biology, School of Basic Medical Sciences, Inner Mongolia Medical University, Hohhot, People's Republic of China.
Inner Mongolia Medical University, Hohhot, People's Republic of China.
Neuropsychiatr Dis Treat. 2020 Apr 20;16:1015-1022. doi: 10.2147/NDT.S248356. eCollection 2020.
Cerebral arteriovenous malformation (cAVM) is a disease characterized by the angiogenesis and remodeling of veins. However, whether vascular endothelial cells (ECs) exhibit morphological and functional changes during cAVM remains unclear. This study aimed to investigate the role of ECs in the pathogenesis of cAVM.
Rat model of cAVM was established by anastomosing the common carotid artery with the external jugular vein. The digital subtraction angiography (DSA), HE, Masson and immunohistochemical staining were performed to evaluate the model. ECs were isolated from AVM rat model or control rats, and characterized by MTT, cell scratch, and tube formation assays. The secretion of vascular endothelial growth factor (VEGF) was detected by ELISA.
AVM rat model showed typical pathological characteristics of cAVM. In addition, the proliferation, migration and tube formation abilities of ECs of arterialized vein (AV-ECs) were significantly better than those of ECs of normal vein (NV-ECs). Moreover, the levels of secreted VEGF were significantly higher in AV-ECs than in NV-ECs.
AV-ECs isolated from AVM rat model showed increased proliferation, migration and angiogenesis and may be potential target for the treatment of cAVM.
脑动静脉畸形（cAVM）是一种以静脉血管生成和重塑为特征的疾病。然而，在cAVM过程中血管内皮细胞（ECs）是否表现出形态和功能变化仍不清楚。本研究旨在探讨ECs在cAVM发病机制中的作用。
通过将颈总动脉与颈外静脉吻合建立cAVM大鼠模型。采用数字减影血管造影（DSA）、苏木精-伊红（HE）染色、Masson染色和免疫组织化学染色对模型进行评估。从AVM大鼠模型或对照大鼠中分离ECs，并通过MTT法、细胞划痕实验和管腔形成实验进行鉴定。采用酶联免疫吸附测定（ELISA）检测血管内皮生长因子（VEGF）的分泌。
AVM大鼠模型表现出cAVM典型的病理特征。此外，动脉化静脉的ECs（AV-ECs）的增殖、迁移和管腔形成能力明显优于正常静脉的ECs（NV-ECs）。而且，AV-ECs中VEGF的分泌水平明显高于NV-ECs。
从AVM大鼠模型中分离出的AV-ECs表现出增殖、迁移和血管生成增加，可能是cAVM治疗的潜在靶点。
