Sumi Toshiko, Stefaneanu Lucia, Kovacs Kalman, Asa Sylvia L, Rindi Guido
Departments of Pathology, St. Michael's Hospital, University of Toronto, Toronto, Ontario, Canada.
Mount Sinai Hospital, Toronto, Ontario, Canada.
Endocr Pathol. 1993 Jun;4(2):95-99. doi: 10.1007/BF02914458.
In many human cancers, p53 gene mutations are frequently occurring genetic abnormalities, which may be detected by immunohistochemical staining for p53 protein. In the present study, p53 immunoreactivity was investigated in formalin-fixed, paraffin-embedded tissues from human and animal pituitary tumors, using the avidin-biotin-peroxidase complex technique. No p53 was detected in 3 nontumorous human adenohypophyses or in 40 human pituitary tumors including 5 GH cell adenomas, 10 PRL cell adenomas, 2 mixed GH cell-PRL cell adenomas, 2 acidophil stem cell adenomas, 8 ACTH cell adenomas, 1 TSH cell adenoma, 1 FSH/LH cell adenoma, 5 null cell adenomas, 5 oncocytomas, and 1 plurihormonal adenoma. Twenty nontumorous and hyperplastic pituitaries of hGRH transgenic mice and 8 tumors in these transgenic animals were immunonegative for p53. All pituitary tumors found in AVP/SV40 transgenic mice contained p53 immunoreactivity in the nuclei, while the nontumorous adenohypophysis of one such transgenic mouse was negative. It can be concluded that p53 mutations are apparently not involved in the pathogenesis of human pituitary adenomas or of the pituitary tumors which develop in hGRH transgenic mice. However, pituitary tumors in AVP/ SV40 transgenic mice are accompanied by p53 expression.
在许多人类癌症中，p53基因突变是常见的基因异常，可通过p53蛋白的免疫组织化学染色检测到。在本研究中，采用抗生物素蛋白-生物素-过氧化物酶复合物技术，对人和动物垂体肿瘤的福尔马林固定、石蜡包埋组织中的p53免疫反应性进行了研究。在3个非肿瘤性人类腺垂体或40个人类垂体肿瘤中未检测到p53，这些肿瘤包括5个生长激素细胞腺瘤、10个催乳素细胞腺瘤、2个生长激素细胞-催乳素细胞混合腺瘤、2个嗜酸性干细胞腺瘤、8个促肾上腺皮质激素细胞腺瘤、1个促甲状腺激素细胞腺瘤、1个促卵泡激素/促黄体生成素细胞腺瘤、5个无功能细胞腺瘤、5个嗜酸性细胞瘤和1个多激素腺瘤。20个hGRH转基因小鼠的非肿瘤性和增生性垂体以及这些转基因动物中的8个肿瘤对p53免疫阴性。在抗利尿激素/猿猴病毒40转基因小鼠中发现的所有垂体肿瘤细胞核中均含有p53免疫反应性，而其中一只此类转基因小鼠的非肿瘤性腺垂体为阴性。可以得出结论，p53突变显然不参与人类垂体腺瘤或hGRH转基因小鼠中发生的垂体肿瘤的发病机制。然而，抗利尿激素/猿猴病毒40转基因小鼠中的垂体肿瘤伴有p53表达。
