Graduate Program of Agricultural and Livestock Microbiology, Faculty of Agrarian and Veterinary Sciences (FCAV/UNESP), Jaboticabal, São Paulo, Brazil; Department of Veterinary Pathology, Faculty of Agrarian and Veterinary Sciences (FCAV/UNESP), Jaboticabal, São Paulo, Brazil.
Koret School of Veterinary Medicine, The Robert H. Smith Faculty of Agriculture, Food and Environment. The Hebrew University of Jerusalem, Rehovot, Israel.
Acta Trop. 2020 Jul;207:105513. doi: 10.1016/j.actatropica.2020.105513. Epub 2020 May 1.
Worldwide, Bartonella species are known to infect a wide range of mammalian and arthropod hosts, including humans. The current study aimed to investigate the prevalence of Bartonella spp. in synanthropic mammals captured in peri-urban areas from Central-Western and Southern Brazil and their ectoparasites. For this aim, 160 mammals belonging to four species, and 218 associated arthropods were sampled. DNA was extracted and subjected to different Bartonella screening assays. Additionally, blood samples from 48 small rodents were submitted to liquid BAPGM culture followed by qPCR assay and solid culture. Two out of 55 Rattus captured in Santa Catarina state were PCR-positive for Bartonella when targeting the nuoG, 16S, and ITS loci. Sequences showed high homology with Bartonella coopersplainsensis. Conversely, all 48 small rodents, 14 capybaras and 43 opossum DNA samples from animals trapped in Mato Grosso do Sul were Bartonella negative in the HRM real time PCR assays targeting the ITS locus and gltA gene. Additionally, all mammal-associated ectoparasites showed negativity results based on HRM real time PCR assays. The present study showed, for the first time, the occurrence of B. coopersplainsensis in Brazil, shedding some light on the distribution of rats-related Bartonella in South America. In addition, the majority of rodents and marsupials were negative for Bartonella spp. Since B. coopersplainsensis reservoirs - Rattus spp. - are widely dispersed around the globe, their zoonotic potential should be further investigated.
在全球范围内，已知巴尔通体物种感染范围广泛的哺乳动物和节肢动物宿主，包括人类。本研究旨在调查巴西中-西部和南部城郊地区共生哺乳动物及其外寄生虫中巴尔通体属的流行情况。为此，共采集了属于四个物种的 160 只哺乳动物和 218 只相关节肢动物。提取 DNA 并进行不同的巴尔通体筛选检测。此外，还对 48 只小型啮齿动物的血液样本进行液体 BAPGM 培养，然后进行 qPCR 检测和固体培养。在圣卡塔琳娜州捕获的 55 只 Rattus 中有 2 只针对 nuoG、16S 和 ITS 基因座的 PCR 检测呈巴尔通体阳性。序列与 Bartonella coopersplainsensis 具有高度同源性。相反，来自马托格罗索州的 48 只小型啮齿动物、14 只水豚和 43 只负鼠的所有 DNA 样本，以及在 HRM 实时 PCR 检测针对 ITS 基因座和 gltA 基因的所有哺乳动物相关外寄生虫均为阴性。本研究首次在巴西发现了 B. coopersplainsensis，这为了解南美的与大鼠相关的巴尔通体分布情况提供了一些线索。此外，大多数啮齿动物和有袋动物均为巴尔通体属阴性。由于 B. coopersplainsensis 的宿主- Rattus spp. -分布广泛，因此其人畜共患潜力应进一步研究。
