Chemistry Department, College of Science, King Saud University, P. O. Box 2455, Riyadh 11451, Saudi Arabia.
Chemistry Department, College of Science, King Saud University, P. O. Box 2455, Riyadh 11451, Saudi Arabia.
J Chromatogr B Analyt Technol Biomed Life Sci. 2020 Jun 1;1146:122125. doi: 10.1016/j.jchromb.2020.122125. Epub 2020 Apr 25.
High-performance liquid chromatography (HPLC) and solid phase micro membrane tip extraction (SPMMTE) methods are developed for the simultaneous analysis of eleven cardiovascular drugs in human plasma. Iron nanoparticles were obtained by the green method, characterized by XRD, FT-IR, TEM, and EDS and utilized in SPMMTE for sample preparation. The mobile phase used was ammonium acetate buffer-methanol-acetonitrile (65:18:17) with a 1.0 mL/min flow rate at 260 nm detection. Column used was Sunshell C 150 × 4.6 mm, 2.6 µm. The values of k, α, and Rs were ranged from 040 to109.22, 1.20 to 2.67 and 1.0 to 26.18. SPMMTE and HPLC methods were fast, reproducible, precise, robust, economic and rugged for analysis of methyldopa, hydrochlorothiazide, prazosin hydrochloride, furosemide, labetalol, propranolol, valsartan, losartan potassium, diltiazem, irbesartan and spironolactone in human plasma. The recoveries (%) of methyldopa, hydrochlorothiazide, prazosin hydrochloride, furosemide, labetalol, propranolol, valsartan, losartan potassium, diltiazem, irbesartan, and spironolactone were 91.0, 85.2, 92.3, 90.4, 90.1, 85.6, 86.6, 86.2, 85.1, 86.6, and 85.7, respectively. These results showed that SPMMTE and HPLC methods can be applied to test the described drugs in several matrices.
建立了高效液相色谱法（HPLC）和固相微萃取膜探头萃取法（SPMMTE），用于同时分析人血浆中的 11 种心血管药物。采用绿色方法制备纳米铁粒子，通过 XRD、FT-IR、TEM 和 EDS 进行表征，并将其用于 SPMMTE 进行样品制备。采用乙酸铵缓冲液-甲醇-乙腈（65:18:17）作为流动相，流速为 1.0 mL/min，检测波长为 260nm。使用的色谱柱为 Sunshell C150×4.6mm，2.6μm。k、α和 Rs 的值范围分别为 0.40-109.22、1.20-2.67 和 1.0-26.18。SPMMTE 和 HPLC 方法快速、重现性好、精密度高、稳健、经济且耐用，可用于分析人血浆中的甲基多巴、氢氯噻嗪、盐酸普萘洛尔、呋塞米、拉贝洛尔、盐酸普萘洛尔、缬沙坦、氯沙坦钾、盐酸地尔硫卓、厄贝沙坦和螺内酯。甲基多巴、氢氯噻嗪、盐酸普萘洛尔、呋塞米、拉贝洛尔、盐酸普萘洛尔、缬沙坦、氯沙坦钾、盐酸地尔硫卓、厄贝沙坦和螺内酯的回收率（%）分别为 91.0、85.2、92.3、90.4、90.1、85.6、86.6、86.2、85.1、86.6 和 85.7。这些结果表明，SPMMTE 和 HPLC 方法可用于测试几种基质中的描述药物。
