Easterbrook-Smith S B, Vandenberg R J, Alden J R
Department of Biochemistry, University of Sydney, Australia.
Mol Immunol. 1988 Dec;25(12):1331-7. doi: 10.1016/0161-5890(88)90048-x.
The initial rate of formation of insoluble immune complexes from rabbit IgG and ovalbumin was approximately 12 times that for formation of F(ab')2-ovalbumin complexes. At low IgG concns, in the range 0.7-2.7 nM, the formation of insoluble immune complexes was characterised by a lag phase, especially for complexes formed in low antigen excess, compared to antibody excess. Guanidine HCl (at concns up to 0.5 M) and urea (at concns up to 1 M) decreased the initial rates of formation of IgG immune complexes more than F(ab')2 immune complexes. Pre-formed IgG immune complexes were solubilised at lower guanidine HCl concns than were F(ab')2 immune complexes. C1q enhanced the initial rate of formation of IgG immune complexes at C1q:IgG ratios up to 1:1. Higher C1q concns decreased the initial rate of formation of the complexes. Urea (1 M) blocked the C1q mediated enhancement of immune complex formation.
由兔免疫球蛋白G（IgG）和卵清蛋白形成不溶性免疫复合物的初始速率约为形成F(ab')2 - 卵清蛋白复合物速率的12倍。在低IgG浓度（0.7 - 2.7 nM范围内）时，与抗体过量相比，在低抗原过量情况下形成不溶性免疫复合物的过程具有一个延迟期。盐酸胍（浓度高达0.5 M）和尿素（浓度高达1 M）对IgG免疫复合物初始形成速率的降低作用比对F(ab')2免疫复合物的作用更大。预先形成的IgG免疫复合物比F(ab')2免疫复合物在更低的盐酸胍浓度下就会溶解。在C1q:IgG比例高达1:1时，C1q会提高IgG免疫复合物的初始形成速率。更高的C1q浓度会降低复合物的初始形成速率。尿素（1 M）会阻断C1q介导的免疫复合物形成增强作用。
