Department of Biochemistry and Molecular Biology, Xinxiang Medical University, Xinxiang, 453003, Henan, China.
International Joint Research Laboratory for Recombinant Pharmaceutical Protein Expression System of Henan, Xinxiang Medical University, Xinxiang, 453003, Henan, China.
Appl Microbiol Biotechnol. 2020 Jul;104(13):5673-5688. doi: 10.1007/s00253-020-10640-w. Epub 2020 May 6.
Human tissue plasminogen activator was the first recombinant therapy protein that successfully produced in Chinese hamster ovary cells in 1986 and approved for clinical use. Since then, more and more therapeutic proteins are being manufactured in mammalian cells, and the technologies for recombinant protein production in this expression system have developed rapidly, with the optimization of both upstream and downstream processes. One of the most promising strategies is expression vector cassette optimization based on the expression vector cassette. In this review paper, these approaches and developments are summarized, and the future strategy on the utilizing of expression cassettes for the production of recombinant therapeutic proteins in mammalian cells is discussed.
人组织纤溶酶原激活物是 1986 年首次在中国仓鼠卵巢细胞中成功生产的重组治疗蛋白，并获得临床批准。此后，越来越多的治疗性蛋白在哺乳动物细胞中生产，该表达系统中重组蛋白生产的技术也迅速发展，上下游工艺都得到了优化。最有前途的策略之一是基于表达载体盒对表达载体盒进行优化。本文综述了这些方法和进展，并讨论了利用哺乳动物细胞中表达盒生产重组治疗性蛋白的未来策略。
