Charles-Bruneau Cancer Center, CHU Sainte-Justine Research Center, University of Montreal, Montreal, Quebec, H3T 1C5, Canada.
Department of Pediatrics, Faculty of Medicine, University of Montreal, Montreal, Quebec, H3T 1J4, Canada.
Pharmacogenomics. 2020 Jun;21(8):541-547. doi: 10.2217/pgs-2019-0195. Epub 2020 May 6.
To evaluate the association between human leukocyte antigen (HLA) alleles and native asparaginase hypersensitivity (AH) in children with acute lymphoblastic leukemia (ALL) who received Dana-Farber Cancer Institute treatment protocols. , and alleles were retrieved from available whole exome sequencing data of a subset of childhood ALL patients from Quebec ALL cohort and analyzed for an association with AH. PCR assay was developed to analyze associated alleles in the entire discovery and replication cohorts. Two alleles in linkage disequilibrium ( and ) were associated with AH. Additional analyses, performed to distinguish between haplotypes with and without allele, showed that the association was dependent on the presence of . This study confirms the implication of , and alleles in developing AH in childhood ALL.
为评估在接受 Dana-Farber 癌症研究所治疗方案的急性淋巴细胞白血病（ALL）患儿中人类白细胞抗原（HLA）等位基因与天然门冬酰胺酶过敏（AH）之间的关联，从魁北克 ALL 队列中儿童 ALL 患者的可用全外显子组测序数据中检索到 、 和 等位基因，并分析其与 AH 的关联。开发了 PCR 检测来分析整个发现和复制队列中的相关等位基因。两个连锁不平衡的等位基因（和）与 AH 相关。进一步进行区分有和无 等位基因的单倍型的分析表明，这种关联取决于 的存在。本研究证实了 、 和 等位基因在儿童 ALL 中引发 AH 的作用。
