Yan Kemin, Wang Xin, Pan Hui, Wang Linjie, Yang Hongbo, Liu Meijuan, Zhu Huijuan, Gong Fengying
Key Laboratory of Endocrinology of National Health Commission, Department of Endocrinology, Peking Union Medical College Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Beijing, China.
Front Pharmacol. 2020 Apr 21;11:482. doi: 10.3389/fphar.2020.00482. eCollection 2020.
Oxidative stress plays an important role in the pathogenesis of obesity and its associated disorders. Safflower yellow (SY) and hydroxysafflor yellow A (HSYA), the natural compounds isolated from , has been found to possess antioxidative and anti-obesity properties. The purpose of the present study is to investigate whether SY and its main component HSYA alleviate obesity by the antioxidant effects.
Diet-induced obese (DIO) mice were treated with 200 mg/kg/d SY or HSYA for 10 weeks. Body weight, fat mass, serum biochemical parameters and superoxide dismutase (SOD) activities were measured. Glucose and insulin tolerance tests were performed. The expression of antioxidant enzymes in liver and adipose tissue were measured. In vitro, HO-induced oxidative stress HepG2 cells and 3T3-L1 adipocytes were treated with SY and HSYA to investigate the direct effects of SY and HSYA on the expression of antioxidant enzymes.
SY and HSYA significantly decreased the body weight gain of DIO mice, and decreased fat mass to 57.8% and 61.6% of the control mice, respectively ( < 0.05). The parameters of glucose metabolism and liver function were improved after SY and HSYA treatment. The hepatic SOD activities and the mRNA levels of antioxidant enzymes in liver and adipose tissue of SY and HSYA treated mice were increased ( < 0.05). Meanwhile, the administration of SY and HSYA on the HO-induced oxidative stress HepG2 cells and adipocytes also increased the expression of the antioxidant factor and antioxidant enzymes to 1.2~3.3 folds of the control cells ( < 0.05).
SY and its main component HSYA could significantly decrease the fat mass, and improve glucose metabolism and liver function in diet-induced obese mice. The beneficial effects of SY and HSYA on obesity and metabolism may be associated with the increased expression of antioxidant enzymes in liver and adipose tissue.
氧化应激在肥胖及其相关疾病的发病机制中起重要作用。从红花中分离出的天然化合物红花黄色素（SY）和羟基红花黄色素A（HSYA）已被发现具有抗氧化和抗肥胖特性。本研究的目的是探讨SY及其主要成分HSYA是否通过抗氧化作用减轻肥胖。
用200mg/kg/d的SY或HSYA处理饮食诱导肥胖（DIO）小鼠10周。测量体重、脂肪量、血清生化参数和超氧化物歧化酶（SOD）活性。进行葡萄糖和胰岛素耐量试验。检测肝脏和脂肪组织中抗氧化酶的表达。在体外，用SY和HSYA处理过氧化氢（HO）诱导氧化应激的HepG2细胞和3T3-L1脂肪细胞，以研究SY和HSYA对抗氧化酶表达的直接影响。
SY和HSYA显著降低了DIO小鼠的体重增加，脂肪量分别降至对照小鼠的57.8%和61.6%（P<0.05）。SY和HSYA处理后，葡萄糖代谢和肝功能参数得到改善。SY和HSYA处理小鼠的肝脏SOD活性以及肝脏和脂肪组织中抗氧化酶的mRNA水平升高（P<0.05）。同时，对HO诱导氧化应激的HepG2细胞和脂肪细胞给予SY和HSYA，也使抗氧化因子和抗氧化酶的表达增加至对照细胞的1.2~3.3倍（P<0.05）。
SY及其主要成分HSYA可显著降低饮食诱导肥胖小鼠的脂肪量，改善葡萄糖代谢和肝功能。SY和HSYA对肥胖和代谢的有益作用可能与肝脏和脂肪组织中抗氧化酶表达增加有关。
