通过 pH 值控制实现通用的高容量蛋白质捕获和释放。
Generic high-capacity protein capture and release by pH control.
机构信息
Department of Chemistry and Chemical Engineering, Chalmers University of Technology, Gothenburg, Sweden.
出版信息
Chem Commun (Camb). 2020 Jun 4;56(44):5889-5892. doi: 10.1039/d0cc01250e. Epub 2020 May 6.
PMID:32373823
Abstract
Techniques for immobilization and release of proteins are of general interest but challenging to develop. Here we show a new method for high-capacity (several μg cm) immobilization of proteins in polyelectrolyte brushes by multivalent hydrogen bonds. Upon increasing pH, the proteins are fully released with preserved structure and activity.
摘要
蛋白质的固定和释放技术具有普遍的研究意义，但开发起来具有挑战性。在这里，我们展示了一种新的方法，通过多价氢键在聚电解质刷中实现高容量（几微克/平方厘米）的蛋白质固定。当 pH 值增加时，蛋白质可以完全释放，且结构和活性得到保留。
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