Tottori Naotomo, Nisisako Takasi
Institute of Innovative Research, Tokyo Institute of Technology, Yokohama, Japan.
Lab Chip. 2020 Jun 2;20(11):1999-2008. doi: 10.1039/d0lc00354a.
This paper proposes microfluidic particle separation by sheath-free deterministic lateral displacement (DLD) with inertial focusing in a single straight input channel. Unlike conventional DLD devices for size-based particle separation, in which sheath streams are used to focus the particles before the solution containing them reaches the DLD arrays, the proposed method uses inertial focusing to align the particles along the middle or the sidewalls of the straight rectangular input channel. The two-stage model of inertial focusing is applied to reduce the length of the side-focusing channel. The proposed method is demonstrated by using it to separate fluorescent polymer particles of diameters 13 and 7 μm, in the process of which the effect of the particle focusing regime on the separation performance is also investigated. Through middle focusing, the method is further used to separate MCF-7 cells (a model of circulating tumor cells (CTCs)) and blood cells, with ∼99.0% capture efficiency achieved.
本文提出了一种在单一笔直输入通道中通过无鞘层确定性侧向位移（DLD）与惯性聚焦相结合的微流控粒子分离方法。与传统的基于尺寸的粒子分离DLD装置不同，在传统装置中，鞘流用于在含有粒子的溶液到达DLD阵列之前使粒子聚焦，而本文提出的方法利用惯性聚焦使粒子沿笔直矩形输入通道的中间或侧壁排列。应用惯性聚焦的两阶段模型来缩短侧向聚焦通道的长度。通过使用该方法分离直径为13μm和7μm的荧光聚合物粒子来证明所提出的方法，在此过程中还研究了粒子聚焦方式对分离性能的影响。通过中间聚焦，该方法进一步用于分离MCF-7细胞（循环肿瘤细胞（CTC）模型）和血细胞，实现了约99.0%的捕获效率。
