Clement A, Sardet A, Chadelat K, Grimfeld A, Tournier G
Department of Pulmonary Pediatrics, Hôpital Trousseau, Paris, France.
Pediatr Pulmonol. 1988;5(4):192-7. doi: 10.1002/ppul.1950050403.
The ability of alveolar macrophages (AM) to release hydrogen peroxide (H2O2), an indicator of AM function, was studied in five children with the acquired immunodeficiency syndrome (AIDS) related complex and, for comparison, in 11 children without disorders of the lung parenchyma. In the AIDS-related complex group, pulmonary manifestations were mild, and lung involvement was suspected by moderate clinical and/or radiological features. None had a past history of opportunistic infections; neither did any have lymphopenia. Cytologic study of the bronchoalveolar lavage (BAL) fluid revealed increased cellularity with increased percentage of lymphocytes. The study of H2O2 release was performed on unstimulated AM and on AM stimulated by phorbol myristate acetate (PMA). Under both experimental conditions, the amount of H2O2 accumulated in the medium was significantly increased in the group with AIDS-related complex (P less than 0.001). As no enhanced oxidative activity has been reported in AM from patients with full-blown AIDS, an increased ability of AM to release oxygen metabolites from children with AIDS-related complex may reflect an initial and temporary step in the course of the LAV/HTLV-III pulmonary disease. Determining AM activation might be a reliable method of assessing the evolution of lung disorder in AIDS.
研究了5例患获得性免疫缺陷综合征（AIDS）相关综合征儿童的肺泡巨噬细胞（AM）释放过氧化氢（H2O2）的能力，这是AM功能的一个指标，并以11例无肺实质疾病的儿童作为对照。在AIDS相关综合征组中，肺部表现轻微，根据中度临床和/或放射学特征怀疑有肺部受累。无一例有机会性感染病史；也无淋巴细胞减少症。支气管肺泡灌洗（BAL）液的细胞学研究显示细胞增多，淋巴细胞百分比增加。对未刺激的AM和经佛波酯肉豆蔻酸酯（PMA）刺激的AM进行了H2O2释放研究。在两种实验条件下，AIDS相关综合征组培养基中积累的H2O2量均显著增加（P<0.001）。由于尚未报道过患典型AIDS患者的AM有增强的氧化活性，AIDS相关综合征儿童的AM释放氧代谢产物能力的增加可能反映了淋巴细胞性间质性肺炎/人嗜T淋巴细胞病毒III型（LAV/HTLV-III）肺部疾病病程中的一个初始和暂时阶段。确定AM的激活可能是评估AIDS肺部疾病演变的一种可靠方法。
