The National Research Centre for the Working Environment, Copenhagen, Denmark.
Division of Diet, Disease Prevention and Toxicology, National Food Institute, Technical University of Denmark, Kongens Lyngby, Denmark.
Nanotoxicology. 2020 Jun;14(5):711-724. doi: 10.1080/17435390.2020.1755468. Epub 2020 May 6.
Prenatal particle exposure has been shown to increase allergic responses in offspring. Carbon nanotubes (CNTs) possess immunomodulatory properties, but it is unknown whether maternal exposure to CNTs interferes with offspring immune development. Here, C57Bl/6J female mice were intratracheally instilled with 67 of μg multiwalled CNTs on the day prior to mating. After weaning, tolerance and allergy responses were assessed in the offspring. Offspring of CNT-exposed (CNT offspring) and of sham-exposed dams (CTRL offspring) were intranasally exposed to ovalbumin (OVA) once weekly for 5 weeks to induce airway mucosal tolerance. Subsequent OVA sensitization and aerosol inhalation caused low or no OVA-specific IgE production and no inflammation. However, the CNT offspring presented with significantly lower OVA-specific IgG1 levels than CTRL offspring. In other groups of 5-week-old offspring, low-dose sensitization with OVA and subsequent OVA aerosol inhalation led to significantly lower OVA-specific IgG1 production in CNT compared to CTRL offspring. OVA-specific IgE and airway inflammation were non-significantly reduced in CNT offspring. The immunomodulatory effects of pre-gestational exposure to multiwalled CNTs were unexpected, but very consistent. The observations of suppressed antigen-specific IgG1 production may be of importance for infection or vaccination responses and warrant further investigation.
产前颗粒暴露已被证明会增加后代的过敏反应。碳纳米管（CNTs）具有免疫调节特性，但尚不清楚母体暴露于 CNTs 是否会干扰后代的免疫发育。在这里，C57Bl/6J 雌性小鼠在交配前一天通过气管内滴注 67μg 多壁 CNTs。断乳后，评估后代的耐受和过敏反应。暴露于 CNT 的后代（CNT 后代）和假暴露于 CNT 的母体（CTRL 后代）的后代每周通过鼻腔内暴露于卵清蛋白（OVA）一次，持续 5 周，以诱导气道粘膜耐受。随后的 OVA 致敏和雾化吸入导致低或无 OVA 特异性 IgE 产生和无炎症。然而，CNT 后代的 OVA 特异性 IgG1 水平明显低于 CTRL 后代。在另一组 5 周龄后代中，用 OVA 进行低剂量致敏，随后进行 OVA 雾化吸入，导致 CNT 后代的 OVA 特异性 IgG1 产生明显低于 CTRL 后代。OVA 特异性 IgE 和气道炎症在 CNT 后代中无显著降低。妊娠前暴露于多壁 CNTs 的免疫调节作用出人意料，但非常一致。抗原特异性 IgG1 产生抑制的观察结果可能对感染或疫苗接种反应很重要，值得进一步研究。
