Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture and Rural Affairs, Jimei University, Xiamen 361021, China.
Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture and Rural Affairs, Jimei University, Xiamen 361021, China.
Gene. 2020 Jul 30;749:144754. doi: 10.1016/j.gene.2020.144754. Epub 2020 May 4.
DNA methylation is an essential epigenetic modification that significantly regulates gene expression during development and differentiation. In this study, genome-wide methylation analysis of different gonads of the large yellow croaker was performed using whole-genome bisulfite sequencing (WGBS), which has characterized DNA methylation patterns in gonad tissue and identified candidate regions for future studies. Clustering analysis revealed that male and neomale methylation patterns were close compared to female. Based on KEGG pathway analysis of differentially methylated genes, we obtained signaling pathways related to gonadal development. We further investigated the methylation status of previously reported sex determination genes, and found that these genes showed different methylation status in three types of gonads, which may provide important clues to reveal the sex determination genes in the large yellow croaker. Furthermore, combined with transcriptome analysis, we found 7 sex-related genes in three comparison groups where expression negatively correlated with methylation.
DNA 甲基化是一种重要的表观遗传修饰，在发育和分化过程中显著调控基因表达。本研究采用全基因组亚硫酸氢盐测序（WGBS）对大黄鱼不同性腺进行了全基因组甲基化分析，该方法描述了性腺组织中的 DNA 甲基化模式，并鉴定了未来研究的候选区域。聚类分析显示，与雌性相比，雄性和拟雄性的甲基化模式较为接近。基于差异甲基化基因的 KEGG 通路分析，我们获得了与性腺发育相关的信号通路。我们进一步研究了先前报道的性别决定基因的甲基化状态，发现这些基因在三种性腺中表现出不同的甲基化状态，这可能为揭示大黄鱼性别决定基因提供重要线索。此外，我们结合转录组分析，在三个比较组中发现了 7 个与性别相关的基因，这些基因的表达与甲基化呈负相关。
