Li Xiao-Bing, He Jie, Ma Rong-Rong, Sun Fu-Ying, Wu Wen-Xin, Luo Hua-Ming, Bai Lu-Huai, Qian Dong
College of Marine Sciences, Ningbo University, Ningbo 315823, China.
Taizhou Extention Station for Fishery Technique, Taizhou 315800, China.
Animals (Basel). 2022 Apr 29;12(9):1145. doi: 10.3390/ani12091145.
(Myxosporea: Multivalvulida) parasites are critical pathogens in marine and freshwater fish associated with significant economic losses and reduced market prices caused by post-mortem myoliquefaction or numerous cysts on muscles. In the present study, large yellow croakers infected by were found during fish disease surveillance in China in November 2020 and used for morphological observation and characterization using light DIC microscopy and electron microscopy. Numerous creamy-white oval plasmodia were observed in muscles and on the surface of brain cartilage, gill arches, and serosal surfaces. The spores were considerably longer and thicker than previously reported , with protruding polar filaments (PFs) in the mature spores, fingertip-shaped apical projections (APs), and polar capsules. Phylogenetic analyses with SSU rDNA, LSU rDNA, and mitochondrial DNA showed that the -infected sample (LcK-2020) had the highest similarity to reported in Japan. Based on the morphological characterization and phylogenetic analysis, it could be concluded that the sample LcK-2020 was infected by , which would be the first report of infection in large yellow croaker in China.
（粘孢子虫纲：多瓣虫目）寄生虫是海洋和淡水鱼类中的关键病原体，会导致严重的经济损失，以及因死后肌肉液化或肌肉上出现大量囊肿而使市场价格降低。在本研究中，2020年11月在中国进行鱼类疾病监测期间发现了感染[寄生虫名称未给出]的大黄鱼，并使用光学微分干涉差显微镜和电子显微镜对其进行形态学观察和特征描述。在肌肉以及脑软骨表面、鳃弓和浆膜表面观察到大量乳白色椭圆形的孢质团。孢子比先前报道的长得多且厚得多，成熟孢子中有突出的极丝（PFs）、指尖状顶端突起（APs）和极囊。基于小亚基核糖体DNA（SSU rDNA）、大亚基核糖体DNA（LSU rDNA）和线粒体DNA的系统发育分析表明，感染[寄生虫名称未给出]的样本（LcK - 2020）与日本报道的[寄生虫名称未给出]相似度最高。基于形态学特征和系统发育分析，可以得出结论，样本LcK - 2020感染了[寄生虫名称未给出]，这将是中国大黄鱼感染[寄生虫名称未给出]的首次报道。
