Wintour E M, Lingwood B E, Towstoless M K
Department of Physiology, University of Melbourne, Parkville, Australia.
Placenta. 1988 Nov-Dec;9(6):599-606. doi: 10.1016/0143-4004(88)90003-3.
The passive permeability to 36Cl of isolated pieces of amnion (112), amniochorion (41) and allantois (54) from 55 pregnant ewes was studied in vitro. There was a significant decrease in the permeability coefficient (K) with increasing age in both amnion and allantois when samples from two age groups (72 to 109; 110 to 147 days of gestation) were analysed. There was a highly significant correlation (P less than 0.001) between amnion permeability and amniotic fluid [Cl-], but none between allantoic permeability and allantoic fluid [Cl-]. Amniotic fluid osmolality decreased significantly over the last third of gestation and was significantly correlated with amniotic fluid [Cl-]. Allantoic fluid osmolality increased with age and did not correlate with allantoic fluid [Cl-]. The anion exchange blocker, SITS, significantly decreased K in the amnion in the younger age group, suggesting that anion exchange through cell membranes may be one route of Cl- movement through the young amnion. The results suggest that the permeability of the amnion may play a role in the regulation of the composition of amniotic but not allantoic fluid in sheep.
对来自55只怀孕母羊的羊膜（112份）、羊膜绒毛膜（41份）和尿囊（54份）离体组织的36Cl被动通透性进行了体外研究。当分析两个年龄组（妊娠72至109天；110至147天）的样本时，羊膜和尿囊的通透性系数（K）均随年龄增长而显著降低。羊膜通透性与羊水[Cl-]之间存在高度显著相关性（P小于0.001），但尿囊通透性与尿囊液[Cl-]之间无相关性。羊水渗透压在妊娠最后三分之一期间显著降低，且与羊水[Cl-]显著相关。尿囊液渗透压随年龄增长而升高，与尿囊液[Cl-]无相关性。阴离子交换阻滞剂SITS显著降低了较年轻年龄组羊膜中的K，这表明通过细胞膜的阴离子交换可能是Cl-通过年轻羊膜移动的一条途径。结果表明，羊膜的通透性可能在绵羊羊水而非尿囊液成分的调节中起作用。
