Ning Chuntao, Li Rongtian, Chen Limei, Jin Tong, Qiu Jingjing, Liu Zhixia, Su Zihao, Lin Jingming
Department of Pharmacy, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China.
Guangdong Provincial Hospital for Occupational Disease Prevention and Treatment, Guangzhou 510399, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2020 Feb 29;40(2):203-210. doi: 10.12122/j.issn.1673-4254.2020.02.11.
To assess the performance of high-performance liquid chromatography (HPLC) combined with capillary monolithic column molecularly imprinted with metal organic frame (UiO-66-NH@MIPs capillary monolithic column) for enrichment, purification and detection of Ponceau 4R in .
UiO-66-NH@MIPs monolithic columns were prepared via polymerization, and the adsorption properties and morphology of the columns were characterized by HPLC, scanning electron microscopy (SEM) and infrared (IR) spectral analysis. HPLC with the prepared columns was performed for detecting the content of Ponceau 4R in samples.
The UiO-66-NH@MIPs system showed a good linearity for detecting Ponceau 4R over the concentration range of 0.1-10.0 μg/mL with a correlation coefficient > 0.9999 and a detection limit (S/N=3) of 2.7×10-4 μg/mL. The mean recovery of Ponceau 4R in samples ranged from 82.60% to 105.56%, and the intra-day and inter-day relative standard deviation (RSD) values ranged from 2.4% to 3.4%. The recycling experiment showed that the system could be reused for sensitive detection of Ponceau 4R in . The capacity of UiO-66-NH@MIPs column was 0.178 μg/mg, which was superior to that of other monolithic columns (0.089, 0.080, and 0.111 μg/ mg), demonstrating that the addition of UiO-66-NH increased the adsorption capacity of the system. Under the optimized conditions, the UiO-66-NH@MIPs-HPLC system had an enrichment factor of over 73 folds with obviously reduced interference by the impurity peaks.
The UiO-66-NH@MIPs column-HPLC system has much better performance for enrichment, purification and detection of Ponceau 4R in than direct HPLC.
评估高效液相色谱（HPLC）结合金属有机框架分子印迹毛细管整体柱（UiO - 66 - NH@MIPs毛细管整体柱）对[具体物质]中丽春红4R的富集、纯化及检测性能。
通过[具体聚合方式]制备UiO - 66 - NH@MIPs整体柱，采用高效液相色谱、扫描电子显微镜（SEM）和红外（IR）光谱分析对柱的吸附性能和形态进行表征。使用制备的柱子通过高效液相色谱检测[具体样品]中丽春红4R的含量。
UiO - 66 - NH@MIPs体系在0.1 - 10.0 μg/mL浓度范围内检测丽春红4R具有良好的线性，相关系数>0.9999，检测限（S/N = 3）为2.7×10⁻⁴ μg/mL。[具体样品]中丽春红4R的平均回收率为82.60%至105.56%，日内和日间相对标准偏差（RSD）值为2.4%至3.4%。循环实验表明该体系可重复用于[具体样品]中丽春红4R的灵敏检测。UiO - 66 - NH@MIPs柱的容量为0.178 μg/mg，优于其他整体柱（0.089、0.080和0.111 μg/mg），表明添加UiO - 66 - NH提高了体系的吸附容量。在优化条件下，UiO - 66 - NH@MIPs - HPLC体系的富集因子超过73倍，杂质峰干扰明显降低。
UiO - 66 - NH@MIPs柱 - HPLC体系在[具体物质]中丽春红4R的富集、纯化及检测方面比直接高效液相色谱具有更好的性能。
