Ni Hong, Ding Haihu, Tao Jing, Wang Yuanyuan, Tao Mingfei, Huang Li
Department of Functional Experiment Center, Bengbu Medical College, Bengbu 233030, China.
Department of Pathophysiology, Bengbu Medical College, Bengbu 233030, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2020 Feb 29;40(2):262-267. doi: 10.12122/j.issn.1673-4254.2020.02.19.
To investigate effect of upregulated touch sensation induced by olfactory deprivation on action potentials and ankyrin-G expression in the glutamatergic neurons in the barrel cortex of mice.
Chloroform (40 μL) was dropped into the unilateral nasal cavity to induce olfactory deprivation in 40 C57 mice (12 days old), whose glutamatergic neurons were genetically labeled with yellow fluorescent protein (YFP). Behavioral experiments were carried out to assess the effects of olfactory deprivation on whisker tactile of the mice. The action potentials of the glutamatergic neurons in the barrel cortex on the side with or without chloroform treatment (olfactory deprivation group and control group, respectively) were recorded by patch-clamp electrophysiological recording, and ankyrin-G expression in the proximal axonal segment of the neurons was detected with immunohistochemistry.
Compared with those on the control side, the inter-spike intervals of the barrel glutamatergic neurons were significantly decreased and the absolute refractory periods were significantly shortened on the side with olfactory deprivation ( < 0.01); the expression of ankyrin-G was also significantly increased in the proximal axonal segment of the glutamatergic neurons in the barrel cortex on the side with olfactory deprivation ( < 0.01).
Olfaction deprivation induces up-regulation of touch sensation in mice possibly as a result of functional enhancement of the glutamatergic neurons and increased ankyrin-G expression in the barrel cortex.
研究嗅觉剥夺诱导的触觉增强对小鼠桶状皮质谷氨酸能神经元动作电位及锚蛋白-G表达的影响。
将40只12日龄C57小鼠单侧鼻腔滴入40 μL氯仿以诱导嗅觉剥夺，这些小鼠的谷氨酸能神经元用黄色荧光蛋白（YFP）进行基因标记。进行行为实验以评估嗅觉剥夺对小鼠触须触觉的影响。采用膜片钳电生理记录法记录氯仿处理侧（嗅觉剥夺组）和未处理侧（对照组）桶状皮质谷氨酸能神经元的动作电位，并用免疫组织化学法检测神经元近端轴突段锚蛋白-G的表达。
与对照侧相比，嗅觉剥夺侧桶状谷氨酸能神经元的峰间间期显著缩短，绝对不应期显著缩短（<0.01）；嗅觉剥夺侧桶状皮质谷氨酸能神经元近端轴突段锚蛋白-G的表达也显著增加（<0.01）。
嗅觉剥夺可能通过增强谷氨酸能神经元功能及增加桶状皮质中锚蛋白-G的表达，诱导小鼠触觉上调。
