Chen Zhiheng, Yang Li, Yi Cuiqing, Liu Jun, Sun Ling
Center of Reproductive Medicine, Guangzhou Women and Children's Medical Center, Guangzhou 510623, China.
Prenatal Diagnosis Center, Guangzhou Women and Children's Medical Center, Guangzhou 510623, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2020 Jan 30;40(1):73-78. doi: 10.12122/j.issn.1673-4254.2020.01.12.
To investigate the association of chromosomal polymorphisms with multinucleated embryos in infertile couples undergoing fertilization-intracytoplasmic sperm injection (IVF-ICSI).
This retrospective case-control study was conducted among 1145 infertile couples undergoing their first IVF/ICSI cycles. According to their karyotype, the couples were divided into chromosomal polymorphism group and control group, and the former group was divided into 3 subgroups: inversion group, D and G genome polymorphic group and 1, 9, and 16 qh+group. The blastomere multinucleation rate, clinical pregnancy rate and live birth rate were compared between the groups.
Of the total of 1145 couples, 139 (6.10%) had chromosomal polymorphisms at least in one partner. No significant differences were found in female age, BMI, basal FSH level, total gonadotropin dose, E level on day of HCG, number of oocytes retrieved, fertilization rate, top quality embryo rate, clinical pregnancy rate or live birth rate among the groups ( > 0.05). The multinuclear rate of the embryos in couples with pericentric inversion of chromosomes 1, 9, and Y chromosomes and those with D and G genome polymorphisms were 8.23% and 4.65%, respectively, significantly higher than that in the control group (2.69%; < 0.05); the multinuclear rate of the embryos was 2.77% in 1, 9, and 16 qh+ group, similar with that in the control group ( > 0.05).
Infertile couples with pericentric inversion of chromosomes 1, 9, and Y chromosomes and in those with D and G genome polymorphism are at higher risks of blastomere multinucleation in IVF- ICSI cycles; 1, 9, and 16 qh + polymorphisms do not increase the rate of blastomere multinucleation of the embryos.
探讨染色体多态性与接受卵胞浆内单精子注射（IVF-ICSI）的不孕夫妇多核胚胎之间的关联。
本回顾性病例对照研究在1145对接受首次IVF/ICSI周期治疗的不孕夫妇中进行。根据核型，将夫妇分为染色体多态性组和对照组，前一组又分为3个亚组：倒位组、D和G基因组多态性组以及1、9和16号染色体长臂增长（qh+）组。比较各组间的卵裂球多核化率、临床妊娠率和活产率。
在总共1145对夫妇中，139对（6.10%）至少一方存在染色体多态性。各组间女性年龄、体重指数、基础促卵泡激素水平、总促性腺激素剂量、人绒毛膜促性腺激素（HCG）日雌激素（E）水平、获卵数、受精率、优质胚胎率、临床妊娠率或活产率均无显著差异（P>0.05）。染色体1、9和Y染色体臂间倒位以及D和G基因组多态性夫妇的胚胎多核率分别为8.23%和4.65%，显著高于对照组（2.69%；P<0.05）；1、9和16号染色体长臂增长组胚胎的多核率为2.77%，与对照组相似（P>0.05）。
染色体1、9和Y染色体臂间倒位以及D和G基因组多态性的不孕夫妇在IVF-ICSI周期中卵裂球多核化风险较高；1、9和16号染色体长臂增长多态性不会增加胚胎卵裂球多核化率。
