Acuña Francisco, Barbeito Claudio G, Portiansky Enrique L, Ranea Guadalupe, Nishida Fabian, Miglino María A, Flamini Mirta A
Laboratorio de Histología y Embriología Descriptiva, Experimental y Comparada, Cátedra de Histología y Embriología, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata (LHYEDEC-FCV-UNLP), La Plata, Argentina.
Consejo Nacional de Investigaciones Científicas y Técnicas, CCT, La Plata, Argentina.
J Morphol. 2020 Jul;281(7):710-724. doi: 10.1002/jmor.21127. Epub 2020 May 7.
The uterus is an organ with great plasticity due to the morphological and physiological changes it experiences during the estrous cycle and pregnancy. In mammals, pregnancy requires diverse sex hormones, growth factors and cytokines, among others, for promoting uterine remodeling to favor implantation, placentation, and embryo/fetus survival and growth. The hystricognathi rodent Lagostomus maximus (plains viscacha) has a high rate of embryonic resorption. The cranial and middle implants are reabsorbed 25-35 days after intercourse while the caudal embryos continue with their development until two precocial offspring are born. So far, no uterine studies of non-pregnant L. maximus females were performed to determine the possible existence of variations in the organ that could be related to the differential survival of the implants. We used ultrasonography, as well as morphological, morphometric, histochemical, lectinhistochemical, and immunohistochemical methods to study differences in the uterine glands (area), vasculature (area), and musculature (thickness) along the uterine horns in non-pregnant females. Along the uterus, all these structures were in more advanced developmental condition in the caudal region as compared to more anterior positions. These regional variations could be decisive in explaining the reason why only caudal implantations come to term. In contrast, no differences in the in the luminal and glandular epithelial cells, nor in the degree of cell proliferation and apoptosis, and hormonal receptor staining were found. These parameters could be related to implantation along the uterine horns, but not to the differential survival of the implants.
子宫是一个具有很大可塑性的器官，这是由于它在发情周期和怀孕期间经历的形态和生理变化。在哺乳动物中，怀孕需要多种性激素、生长因子和细胞因子等，以促进子宫重塑，有利于着床、胎盘形成以及胚胎/胎儿的存活和生长。豚鼠科啮齿动物草原犬鼠（Lagostomus maximus）的胚胎吸收率很高。头侧和中间的着床胚胎在交配后25 - 35天被吸收，而尾侧胚胎则继续发育，直到产下两只早熟的后代。到目前为止，尚未对未怀孕的草原犬鼠雌性进行子宫研究，以确定该器官中可能存在的与着床胚胎不同存活率相关的变化。我们使用超声检查以及形态学、形态计量学、组织化学、凝集素组织化学和免疫组织化学方法，研究未怀孕雌性子宫角沿线子宫腺体（面积）、脉管系统（面积）和肌肉组织（厚度）的差异。沿着子宫，与更靠前的位置相比，所有这些结构在尾侧区域的发育状况更先进。这些区域差异可能是解释为什么只有尾侧着床能够足月的决定性因素。相比之下，在腔上皮细胞和腺上皮细胞、细胞增殖和凋亡程度以及激素受体染色方面未发现差异。这些参数可能与子宫角沿线的着床有关，但与着床胚胎的不同存活率无关。
