College of Animal Science and Technology, Northwest A&F University, Yangling, P.R. China.
Dairy Cattle Research Center, Shandong Academy of Agricultural Sciences, Jinan, P.R. China.
DNA Cell Biol. 2020 Jul;39(7):1119-1126. doi: 10.1089/dna.2020.5517. Epub 2020 May 5.
() belongs to the NAD-dependent deacetylase family, is more highly expressed than other family members in adipocytes, and plays crucial roles in a wide range of biological processes. However, the mechanisms underlying expression during adipogenesis are poorly studied. In this study, the transcriptional start site (TSS) of was identified and two alternative transcript variants were spliced from . The 5'-regulatory region of was also characterized; no TATA-box or CCAAT-box was presented in the 5'-flanking region. Two cytosine-phosphate diester-guanine (CpG) islands were also identified between nucleotides -563 and +4. A dual-luciferase reporter assay revealed that a 178 base pair sequence upstream from the TSS (+1) was the core promoter of . Results from a site-directed mutagenesis experiment, electrophoretic mobility shift assay, and chromatin immunoprecipitation assay indicated Yin Yang 1 (YY1) to be a positive regulator of bovine in preadipocytes. YY1 is likely to suppress adipogenesis in two different ways by regulating peroxisome proliferator-activated receptor gamma expression. Our results expand the information on the regulatory network of adipogenesis, which is an important basis for improving beef quality, treating obesity, and other related diseases.
() 属于 NAD 依赖的去乙酰化酶家族，在脂肪细胞中比其他家族成员表达更高，在广泛的生物学过程中发挥关键作用。然而，在脂肪生成过程中表达的机制尚未得到充分研究。在这项研究中，确定了 的转录起始位点（TSS），并从 拼接了两种替代的转录变体。 5'调控区也进行了特征描述；在 5'侧翼区没有 TATA 框或 CCAAT 框。在核苷酸 -563 到 +4 之间还鉴定出两个胞嘧啶-磷酸二酯-鸟嘌呤（CpG）岛。双荧光素酶报告基因检测表明，TSS（+1）上游的 178 个碱基序列是 的核心启动子。定点突变实验、电泳迁移率变动分析和染色质免疫沉淀分析的结果表明，Yin Yang 1 (YY1) 是前脂肪细胞中牛 的正向调节因子。YY1 可能通过调节过氧化物酶体增殖物激活受体 γ 的表达，以两种不同的方式抑制脂肪生成。我们的研究结果扩展了脂肪生成调控网络的信息，这是提高牛肉质量、治疗肥胖症和其他相关疾病的重要基础。
