Coughlin R W, Aizawa M, Charles M
Biotechnol Bioeng. 1976 Feb;18(2):199-208. doi: 10.1002/bit.260180206.
A soluble-insoluble form of nicotinamide adenine dinucleotide (NAD+), which can be rendered either soluble or insoluble by simply adjusting the pH, has been prepared by covalently coupling NAD to alginic acid using 1,2,7,8-diepoxyoctane. The NAD bound to the alginic acid showed the coenzymic function in the soluble state and could be collected for further use as precipitate by lowering the pH to below 3. Coupling soluble-insoluble coenzymes with insolubilized apoenzymes is possible in fluidized and fixed-bed reactors.
通过使用1,2,7,8 - 二环氧辛烷将烟酰胺腺嘌呤二核苷酸（NAD⁺）与海藻酸共价偶联，制备了一种可溶 - 不溶形式的烟酰胺腺嘌呤二核苷酸，只需调节pH值即可使其变为可溶或不溶状态。与海藻酸结合的NAD在可溶状态下表现出辅酶功能，通过将pH值降低至3以下，可将其作为沉淀物收集以供进一步使用。在流化床和固定床反应器中，可将可溶 - 不溶辅酶与不溶性脱辅酶偶联。
