Peroni Eleonora, Scali Viola, Balestri Francesco, Cappiello Mario, Mura Umberto, Del Corso Antonella, Moschini Roberta
Ecoverde SpA, via IV Novembre, 55016 Porcari (LU), Italy.
Department of Biology, Biochemistry Unit, University of Pisa, via S. Zeno, 51, 56123 Pisa, Italy.
Antioxidants (Basel). 2020 May 5;9(5):385. doi: 10.3390/antiox9050385.
One of the consequences of the increased level of oxidative stress that often characterizes the cancer cell environment is the abnormal generation of lipid peroxidation products, above all 4-hydroxynonenal. The contribution of this aldehyde to the pathogenesis of several diseases is well known. In this study, we characterized the ADF astrocytoma cell line both in terms of its pattern of enzymatic activities devoted to 4-hydroxynonenal removal and its resistance to oxidative stress induced by exposure to hydrogen peroxide. A comparison with lens cell lines, which, due to the ocular function, are normally exposed to oxidative conditions is reported. Our results show that, overall, ADF cells counteract oxidative stress conditions better than normal cells, thus confirming the redox adaptation demonstrated for several cancer cells. In addition, the markedly high level of NADP-dependent dehydrogenase activity acting on the glutahionyl-hydroxynonanal adduct detected in ADF cells may promote, at the same time, the detoxification and recovery of cell-reducing power in these cells.
癌细胞环境通常具有氧化应激水平升高的特征，其后果之一是脂质过氧化产物异常生成，尤其是4-羟基壬烯醛。这种醛对多种疾病发病机制的作用已广为人知。在本研究中，我们从致力于去除4-羟基壬烯醛的酶活性模式及其对过氧化氢诱导的氧化应激的抗性方面对ADF星形细胞瘤细胞系进行了表征。报告了与晶状体细胞系的比较，由于眼功能，晶状体细胞系通常处于氧化条件下。我们的结果表明，总体而言，ADF细胞比正常细胞能更好地抵抗氧化应激条件，从而证实了几种癌细胞所表现出的氧化还原适应性。此外，在ADF细胞中检测到的作用于谷胱甘肽基-羟基壬烯醛加合物上的NADP依赖性脱氢酶活性显著高水平，可能同时促进这些细胞的解毒和细胞还原能力的恢复。
