Cardosa M J, Noor Sham S, Tio P H, Lim S S
School of Medical Sciences, Universiti Sains Malaysia, Penang.
Southeast Asian J Trop Med Public Health. 1988 Dec;19(4):591-4.
A dot enzyme immunoassay (DEIA) was used to determine the levels of antibody to dengue 3 virus in the acute and convalescent sera of febrile patients with a clinical diagnosis of dengue fever or dengue haemorrhagic fever. The antibody titres were compared with titres determined by the haemagglutination inhibition (HI) test. The results of the study showed that, besides being more simple to perform, the DEIA is in order of magnitude more sensitive than the HI test. Furthermore, the data suggest that it is possible to use a single dilution as a cutoff point to predict with reasonable accuracy, if a patient has had a recent dengue infection. The DEIA test for antibodies to dengue virus is an appropriate technology highly suitable for rapid diagnosis and surveillance in developing countries.
采用斑点酶免疫测定法（DEIA）检测临床诊断为登革热或登革出血热的发热患者急性期和恢复期血清中登革3型病毒抗体水平。将抗体滴度与血凝抑制（HI）试验测定的滴度进行比较。研究结果表明，DEIA除操作更简单外，其灵敏度比HI试验高几个数量级。此外，数据表明，有可能将单一稀释度作为临界值，以合理的准确度预测患者近期是否感染登革热。登革病毒抗体的DEIA检测是一项非常适合发展中国家进行快速诊断和监测的适用技术。
