El-Zeftawy Marwa, Mahmoud Gamal B, Hassan Mervat
Biochemistry Department, Faculty of Veterinary Medicine, New Valley University, New Valley, Egypt.
Biochemistry Department, Faculty of Science, Biological Screening and Preclinical Trial Lab, Alexandria University, Alexandria, Egypt.
Reprod Domest Anim. 2020 Jul;55(7):870-881. doi: 10.1111/rda.13697. Epub 2020 May 26.
Heat stress (HS) occupies huge importance nowadays as it leads to severe economic losses especially in livestock. Preserving sheep against HS is one of the governmental scopes where it represents huge percentage of global ruminant. The present research was conducted to study semen quality, some stress and inflammatory markers in Ossimi rams under both hot and mild climatic conditions. The current study was conducted on selected 46 ram samples divided into two groups during summer and winter. Semen analysis, testosterone (TES), cortisol (COR) and blood glucose (BG) levels, and lipid and protein profiles were done. Concentrations of tumour necrosis factor alpha (TNF-α), tissue inhibitor of metalloproteinase-3 (TIMP-3), nitric oxide (NO), malondialdehyde (MDA) and reduced glutathione (GSH) and specific activity of glutathione peroxidase (GPx) and superoxide dismutase (SOD) were assessed. The results in summer compared to winter revealed significant elevation of total defects and number of dead sperms; however, there was reduction in sperm total motility and concentration and external epididymal tail duct diameter. Histological study of epididymal tail lumen exhibited azoospermia. Further, TES, TIMP-3 and GSH levels were decreased and COR, TNF-α, NO and MDA were raised. Specific activities of GPx and SOD were also declined. Additionally, there was a significant increase in concentrations of BG and lipid profiles except high-density lipoprotein. Our data concluded that there were new insights into TNF-α and TIMP-3 as biomarkers can be used in diagnosis of sheep suffering from HS, but further studies are recommended to do in future work about such aspect.
热应激（HS）如今具有极其重要的意义，因为它会导致严重的经济损失，尤其是在畜牧业中。保护绵羊免受热应激是政府的工作范畴之一，因为绵羊在全球反刍动物中占比很大。本研究旨在探讨炎热和温和气候条件下奥西米公羊的精液质量、一些应激和炎症标志物。本研究在夏季和冬季对46只选定的公羊样本进行，分为两组。进行了精液分析、睾酮（TES）、皮质醇（COR）和血糖（BG）水平以及脂质和蛋白质谱分析。评估了肿瘤坏死因子α（TNF-α）、金属蛋白酶组织抑制剂-3（TIMP-3）、一氧化氮（NO）、丙二醛（MDA）和还原型谷胱甘肽（GSH）的浓度以及谷胱甘肽过氧化物酶（GPx）和超氧化物歧化酶（SOD）的比活性。与冬季相比，夏季的结果显示总缺陷和死精数量显著增加；然而，精子总活力、浓度和附睾外尾管直径降低。附睾尾管腔的组织学研究显示无精子症。此外，TES、TIMP-3和GSH水平降低，COR、TNF-α、NO和MDA升高。GPx和SOD的比活性也下降。此外，BG和脂质谱浓度显著增加，但高密度脂蛋白除外。我们的数据得出结论，对于TNF-α和TIMP-3作为生物标志物有了新的认识，可用于诊断患有热应激的绵羊，但建议在未来的工作中对此方面进行进一步研究。
