Pereira-de-Morais Luís, Silva Andressa de Alencar, da Silva Renata Evaristo Rodrigues, Ferraz Navarro Daniela Maria do Amaral, Melo Coutinho Henrique Douglas, Menezes Irwin Rose Alencar de, Kerntopf Marta Regina, Cunha Francisco Assis Bezerra da, Leal-Cardoso José Henrique, Barbosa Roseli
Regional University of Cariri, Pimenta Campus, 63105-010 Crato, CE, Brazil; State University of Ceará, Itapery Campus, 60741-000 Fortaleza, CE, Brazil.
State University of Ceará, Itapery Campus, 60741-000 Fortaleza, CE, Brazil.
Food Chem. 2020 May 1;325:126923. doi: 10.1016/j.foodchem.2020.126923.
This study aimed to investigate the myorelaxant action of the Dysphania ambrosioides essential oil (EODa) and its major constituent α-terpinene on tracheal smooth muscle isolated from rats. In tracheal smooth muscle ex vivo, in organ baths, isometric contractions recordings were done in order to evaluated the effect of EODa (1-1000 μg/mL) and α-terpinene (1-3000 μg/mL) on the following parameters: basal tone, contractions evoked by potassium (KCl 60 mM), acetylcholine (ACh 10 μM) or serotonin (5-HT 10 μM). The EODa and its major constituent α-terpinene, did not statistically alter basal tone; however, they induced myorelaxant effects on top of contractions induced by KCl, ACh and 5-HT. EODa and α-terpinene also inhibited the contractions induced by barium in presence of High [K] (80 mM). The data suggest that the relaxation induced by these agents is caused by the inhibition of L-type VGCC, inhibiting the inward Ca current through these channels, but does not exclude the possibility of participation of other mechanisms. Results from this study also suggest the EODa, due to their efficacy on relaxation of the respiratory tract, posses a therapeutic potential as a antispasmodic agent for respiratory tract.
本研究旨在探讨土荆芥精油（EODa）及其主要成分α-萜品烯对大鼠离体气管平滑肌的肌松作用。在离体气管平滑肌中，于器官浴槽中进行等长收缩记录，以评估EODa（1 - 1000μg/mL）和α-萜品烯（1 - 3000μg/mL）对以下参数的影响：基础张力、由钾（60mM KCl）、乙酰胆碱（10μM ACh）或5-羟色胺（10μM 5-HT）诱发的收缩。EODa及其主要成分α-萜品烯对基础张力无统计学上的改变；然而，它们在由KCl、ACh和5-HT诱发的收缩基础上诱导了肌松作用。EODa和α-萜品烯在高钾（80mM）存在时也抑制了钡诱发的收缩。数据表明，这些药物诱导的松弛是由L型电压门控钙通道的抑制引起的，抑制了通过这些通道的内向钙电流，但不排除其他机制参与的可能性。本研究结果还表明，EODa因其对呼吸道松弛的功效，具有作为呼吸道解痉剂的治疗潜力。
