Iammarino Marco, Haouet Naceur, Di Taranto Aurelia, Berardi Giovanna, Benedetti Ferdinando, Di Bella Sara, Chiaravalle Antonio Eugenio
Chemistry Department, Istituto Zooprofilattico Sperimentale della Puglia e della Basilicata, Via Manfredonia 20, 71121 Foggia, Italy.
Food Control Laboratory, Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche, Via G. Salvemini 1, 06126 Perugia, Italy.
Food Chem. 2020 Apr 27;325:126937. doi: 10.1016/j.foodchem.2020.126937.
Being an indirect approach, the standard method for the determination of polyphosphates (PPs) in food maybe characterized by high variability which can lead to significant errors in the final measurement. In this study, this method was validated and then compared to the ion chromatography-based approach. Method trueness and measurement uncertainty resulted comparable, while the precision of ion chromatography approach was higher. No "false positive" responses were obtained from ion chromatography analysis, while indirect photometry may cause "false positive" results (5% of analyzed samples). Regarding "false negative" results, this possibility maybe verified for all food types tested and for seafood when using indirect photometry and ion chromatography, respectively. The percentages of "false negative" samples were equal to 23.3% for indirect photometry and 10% for ion chromatography. In conclusion, the indirect photometry is a reliable method especially as "screening technique", while ion chromatography maybe used as a confirmatory technique, successfully.
作为一种间接方法，食品中多磷酸盐（PPs）测定的标准方法可能具有高度变异性，这可能导致最终测量出现重大误差。在本研究中，对该方法进行了验证，然后与基于离子色谱的方法进行了比较。方法的准确性和测量不确定度相当，而离子色谱法的精密度更高。离子色谱分析未获得“假阳性”响应，而间接光度法可能会导致“假阳性”结果（5%的分析样品）。关于“假阴性”结果，使用间接光度法和离子色谱法时，分别对所有测试食品类型和海鲜进行了验证。间接光度法的“假阴性”样品百分比为23.3%，离子色谱法为10%。总之，间接光度法是一种可靠的方法，特别是作为“筛选技术”，而离子色谱法可以成功地用作确证技术。
