Zhu Wenxuan, Zhao Shuhua, Yu Bei, Tao Yucun, Wang Chaoyang, Shi Lei, Zhang Xiufeng, Meng Jing, Wu Chengqiu, Ding Chunguang, Yu Lijia
School of Public Health, Hengyang Medical School, University of South China, Hengyang, 421001, China.
NHC Key Laboratory for Engineering Control of Dust Hazard, National Center for Occupational Safety and Healthy, NHC, Beijing, 102308, China.
Anal Sci. 2025 Mar;41(3):289-296. doi: 10.1007/s44211-024-00697-2. Epub 2024 Dec 26.
We synthesized a squaraine dye (F-0) to develop a method for detecting pyrophosphate (PPi) and alkaline phosphatase (ALP) by modulating the fluorescence of F-0. The fluorescence intensity of the F-0 system was quenched upon the addition of Cu ions; however, it was restored when PPi was introduced due to the formation of a complex between PPi and Cu. Since ALP can hydrolyze PPi, the fluorescence of the system was quenched again upon the addition of ALP. Based on these principles, we established a fluorescent probe that exhibits an "off-on-off" fluorescence response. The detection limits of this method for PPi and ALP were 103 nmol dm and 0.18 U dm, respectively. Moreover, this method demonstrates good selectivity and specificity and can be applied to the detection of PPi in actual samples.
我们合成了一种方酸菁染料（F-0），以开发一种通过调节F-0的荧光来检测焦磷酸（PPi）和碱性磷酸酶（ALP）的方法。加入铜离子后，F-0体系的荧光强度猝灭；然而，由于PPi与铜形成络合物，当引入PPi时荧光恢复。由于ALP可以水解PPi，加入ALP后体系的荧光再次猝灭。基于这些原理，我们建立了一种呈现“关-开-关”荧光响应的荧光探针。该方法对PPi和ALP的检测限分别为103 nmol dm和0.18 U dm。此外，该方法具有良好的选择性和特异性，可应用于实际样品中PPi的检测。
