Department of Environmental Engineering, School of Architecture and Civil Engineering, Chengdu University, Chengdu 610106, China; Botany and Microbiology Department, Faculty of Science, Tanta University, Tanta 31527, Egypt.
Botany and Microbiology Department, Faculty of Science, Tanta University, Tanta 31527, Egypt.
Bioresour Technol. 2020 Aug;310:123462. doi: 10.1016/j.biortech.2020.123462. Epub 2020 Apr 29.
In the present study, a halophilic microalgal species was isolated from a hypersaline lagoon with salinity average of 45.3‰ and identified as Dunaliella salina KSA-HS022. It was further cultivated at a salinity range of 50-250‰, applied directly to batch cultures or through stepwise increase in a semi-continuous culture. The later showed the highest biomass productivity of 0.191 g L d at 125‰, which represented 45.8% higher than the corresponding batch culture (control). Oxidative markers in the control cultures were significantly higher than those of the adapted culture, confirming reduction of oxidative stress by adaptation. In addition, stepwise adaptation showed the highest lipid productivity of 56.5 mg L d at 150‰ (39.9% higher than the corresponding control), which resulted in the highest fatty acid methyl esters productivity. Moreover, stepwise increase of salinity up to 150‰ enhanced the biodiesel characteristics, offering a new route for enhanced biodiesel production at extraordinary salinity levels.
在本研究中，从盐度平均为 45.3‰的高盐泻湖中分离出一种嗜盐微藻物种，并鉴定为盐生杜氏藻 KSA-HS022。进一步在 50-250‰盐度范围内进行培养，直接应用于分批培养或通过半连续培养逐步增加。后者在 125‰时表现出最高的生物量生产力，为 0.191 g L d，比相应的分批培养（对照）高 45.8%。对照培养物中的氧化标志物明显高于适应培养物，证实了适应可以减轻氧化应激。此外，逐步适应在 150‰时表现出最高的脂质生产力，为 56.5 mg L d（比相应的对照高 39.9%），从而实现了最高的脂肪酸甲酯生产力。此外，逐步提高盐度至 150‰增强了生物柴油的特性，为在特殊盐度水平下提高生物柴油生产提供了新途径。
