Westerdijk Fungal Biodiversity Institute, Uppsalalaan 8, 3584, CT, Utrecht, the Netherlands.
Studio: Bacinol 2, Hooikade 13, 2627, AB, Delft, the Netherlands.
Fungal Biol. 2020 May;124(5):509-515. doi: 10.1016/j.funbio.2020.03.009. Epub 2020 Mar 31.
The extreme xerophilic fungus Aspergillus restrictus is used as a model for a large artwork created out of five microscopic pictures in total measuring 80 cm by 624 cm. The artwork is printed on aluminium and located at the entrance of the Westerdijk Institute, Utrecht, The Netherlands. The first picture is made from a colony of the fungus, which has a dimension of 1 cm and the last picture shows details of ornamentation on conidia and phialides of the fungus. The first two pictures of the artwork are made using a unique method of light microscopy in which many hundreds of pictures are made at different focal depths resulting in high detail and resolution of the pictures. For three other pictures, cryo-electron scanning microscopy was used including both a conventional system for lower magnification and a field emission scanning electron microscope for high resolution micrographs. The range of magnification is, at real size, between 78 and 63,000 times. When the observer passes the artwork it acts like a virtual microscope, just by walking past it you zoom-in to the smallest possible details. This coherent increase of magnification of one fungus, with very high quality light- and electron microscopy micrographs, shows different layers of fungal organization and emergent properties. These include the occurrence of secondary outcrops of hyphae and conidiophores in a colony; the formation of a stipe on a thin aerial hyphae; the presence and formation of characteristic structures on stipes, vesicles and phialides and a continuous zone between the forming conidia and phialides.
极端嗜干真菌构巢曲霉被用作一个大型艺术品的模型,该艺术品由总共五张微观图片组成,总面积为 80 厘米乘 624 厘米。该艺术品印在铝板上,位于荷兰乌得勒支的 Westerdijk 研究所入口处。第一张图片是由真菌的一个菌落组成,其尺寸为 1 厘米,最后一张图片显示了真菌分生孢子和梗的装饰细节。该艺术品的前两张图片是使用独特的光学显微镜方法制作的,其中在不同的聚焦深度下制作了数百张图片,从而实现了图片的高细节和高分辨率。对于另外三张图片,则使用了 cryo-electron scanning microscopy,包括用于低倍放大的常规系统和用于高分辨率显微照片的场发射扫描电子显微镜。实际放大倍数范围为 78 倍至 63,000 倍。当观察者经过艺术品时,它就像一个虚拟显微镜一样,只需走过它,你就可以放大到最小的细节。这种对一个真菌的协同放大,结合了非常高质量的光学和电子显微镜显微照片,展示了不同层次的真菌组织和涌现特性。这些特性包括在菌落中出现二次菌丝和梗的露头;在稀薄气生菌丝上形成梗;梗、泡囊和梗上存在和形成特征结构以及形成中的分生孢子和梗之间的连续区域。