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[应用高效液相色谱法和薄层层析法对尿毒症患者血浆中分子质量组分的研究]

[Study of middle molecular weight fraction in the plasma of patients with uremia using high performance liquid chromatography and thin layer chromatography].

作者信息

Kovalishin Ia F, Nikolaev V G, Baran E Ia, Dudar' I A, Iushko L A

出版信息

Vopr Med Khim. 1988 Nov-Dec;34(6):23-6.

PMID:3238937
Abstract

Detection and quantitative estimation of peptides were carried out in the middle molecular fraction of blood plasma from patients with uremia using high performance liquid chromatography and thin-layer chromatography. The middle molecular fraction was separated into four fractions after ultrafiltration through a column of Protein Pak i-60. As low as 40-60 mcg/ml of the peptides were detected in the first and second fractions.

摘要

采用高效液相色谱法和薄层色谱法,对尿毒症患者血浆中的中分子部分进行了肽类物质的检测和定量评估。通过Protein Pak i - 60柱超滤后,中分子部分被分离为四个部分。在第一和第二部分中检测到低至40 - 60微克/毫升的肽类物质。

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