Beijing National Laboratory for Molecular Sciences, Key Laboratory of Organic Solids, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100910, P. R. China.
College of Chemistry, University of Chinese Academy of Sciences, Beijing 100049, P. R. China.
ACS Appl Mater Interfaces. 2020 Jun 3;12(22):24655-24661. doi: 10.1021/acsami.0c07476. Epub 2020 May 21.
Optogenetics holds great potential for precisely altering living cell behavior with the aid of light because of its high temporospatial resolution. However, the light-dependent manner severely limits its applications in deep tissues, particularly to those in the visible region. Here, we propose a wireless charging electrochemiluminescence (ECL) system, featured with long-time delayed luminescence, to remotely activate the light-gated ion channel (channelrhodopsin-2, ChR2) on the living cell membrane, followed by the intracellular influx of Ca ions. Upon wireless charging ECL illumination, the influx of Ca into the living cells triggers strong ion indicator fluorescence, suggesting the successful remote control on ChR2. As such, the wireless charging ECL strategy exhibits great potential to wireless control of optogenetics in deep tissues by implanting a device in vivo.
光遗传学凭借其高时空分辨率，借助光精确地改变活细胞行为的潜力巨大。然而，光依赖性严重限制了其在深层组织中的应用，特别是在可见光区域。在这里，我们提出了一种无线充电电化学发光（ECL）系统，其具有长时间延迟发光的特点，可远程激活活细胞膜上的光门控离子通道（通道视紫红质-2，ChR2），随后细胞内流入 Ca 离子。在无线充电 ECL 照射下，Ca 流入活细胞会引发强离子指示剂荧光，表明 ChR2 的远程控制取得了成功。因此，通过在体内植入设备，无线充电 ECL 策略有望实现深层组织中光遗传学的无线控制。
