Department of Laboratory Medicine, Changzhou TCM Hospital, Changzhou, China.
Department of Obstetrics and Gynecology, Wuxi Hospital of Integrated Traditional Chinese and Western Medicine, Affiliated Hospital of Jiangnan University, Wuxi, China.
Gynecol Endocrinol. 2020 Dec;36(12):1051-1056. doi: 10.1080/09513590.2020.1760817. Epub 2020 May 12.
Our study aimed to explore the relationship between leptin and IFN-γ in PCOS patients, and confirmed the effect of leptin-induced IFN-γ on granulosa cells furtherly.
29 patients with PCOS and 36 healthy controls were enrolled. Leptin level and the proportion of Th1 cells were detected and association between them were analyzed. Meanwhile, peripheral blood mononuclear cells (PBMCs) isolated from PCOS patients were treated with leptin and then the proportion of Th1 was analyzed. Besides that, the apoptotic level of KGN cells was monitored after IFN-γ treatment.
In the circulation of PCOS patients, leptin level dramatically increased compared with controls. And, this was associated with upregulated Th1 cells proportion and IFN-γ level. , Th1 cells proportion increased after leptin treated PBMCs from PCOS patients. Furthermore, for KGN cells, the percentage of live cells decreased and later apoptosis cells increased after IFN-γ treatment.
Our results indicated that leptin takes part in process of PCOS inducing expression of IFN-γ. Our findings highlight the importance of the connection between leptin and inflammation in PCOS and provide new insights therapeutic strategy for this disease.
本研究旨在探讨多囊卵巢综合征（PCOS）患者瘦素与干扰素-γ（IFN-γ）之间的关系，并进一步证实瘦素诱导的 IFN-γ对颗粒细胞的影响。
纳入 29 例 PCOS 患者和 36 名健康对照者，检测瘦素水平和 Th1 细胞比例，并分析两者之间的相关性。同时，用瘦素处理 PCOS 患者的外周血单个核细胞（PBMCs），然后分析 Th1 细胞的比例。此外，用 IFN-γ处理 KGN 细胞后，监测其凋亡水平。
与对照组相比，PCOS 患者循环中的瘦素水平显著升高。并且，这与 Th1 细胞比例和 IFN-γ水平的上调有关。用瘦素处理 PCOS 患者的 PBMCs 后，Th1 细胞比例增加。此外，对于 KGN 细胞，IFN-γ处理后活细胞的百分比降低，凋亡细胞的比例增加。
我们的研究结果表明，瘦素参与了 PCOS 诱导 IFN-γ表达的过程。我们的发现强调了瘦素与 PCOS 中炎症之间的联系的重要性，并为该疾病的治疗策略提供了新的见解。
