Departamento de Biologia, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, 14040-901, Brasil.
Facultad de Ciencias de La Tierra Y Agua, Universidad Regional Amazónica Ikiam, km 7, vía a Muyuna, Tena, Napo, Ecuador.
Bull Environ Contam Toxicol. 2020 Jun;104(6):755-762. doi: 10.1007/s00128-020-02872-3. Epub 2020 May 11.
We examined copper accumulation in the hemolymph, gills and hepatopancreas, and hemolymph osmolality, Na and Cl concentrations, together with gill Na/K-ATPase and carbonic anhydrase activities, after dietary copper delivery (0, 100 or 500 Cu µg g) for 12 days in a fiddler crab, Minuca rapax. In contaminated crabs, copper concentration decreased in the hemolymph and hepatopancreas, but increased in the gills. Hemolymph osmolality and gill Na/K-ATPase activity increased while hemolymph [Na] and [Cl] and gill carbonic anhydrase activity decreased. Excretion likely accounts for the decreased hemolymph and hepatopancreas copper titers. Dietary copper clearly affected osmoregulatory ability and hemolymph Na and Cl regulation in M. rapax. Gill copper accumulation decreased carbonic anhydrase activity, suggesting that dietary copper affects acid-base balance. Elevated gill Na/K-ATPase activity appears to compensate for the ion-regulatory disturbance. These effects of dietary copper illustrate likely impacts on semi-terrestrial species that feed on metal-contaminated sediments.
我们研究了在饮食铜暴露（0、100 或 500μgCu g）12 天后，扇蟹（Minuca rapax）血淋巴、鳃和肝胰腺中的铜积累，以及血淋巴渗透压、Na 和 Cl 浓度，同时还研究了鳃 Na/K-ATP 酶和碳酸酐酶的活性。在受污染的螃蟹中，铜在血淋巴和肝胰腺中的浓度降低，但在鳃中的浓度增加。血淋巴渗透压和鳃 Na/K-ATP 酶活性增加，而血淋巴 [Na]和 [Cl]和鳃碳酸酐酶活性降低。排泄可能是血淋巴和肝胰腺铜含量降低的原因。饮食铜明显影响了 M. rapax 的渗透调节能力和血淋巴 Na 和 Cl 调节。鳃铜积累降低了碳酸酐酶活性，表明饮食铜影响酸碱平衡。高鳃 Na/K-ATP 酶活性似乎补偿了离子调节紊乱。饮食铜的这些影响表明，它可能对以受金属污染沉积物为食的半陆生物种产生影响。
