Department of Obstetrics and Gynecology, University of Health Sciences, Etlik Zübeyde Hanım Women’s Health Training and Research Hospital, Ankara, Turkey
Department of Gynecology and Obstetrics, Faculty of Veterinary Medicine, Ankara University, Ankara, Turkey
Turk J Med Sci. 2020 Aug 26;50(5):1399-1408. doi: 10.3906/sag-2002-117.
BACKGROUND/AIM: To evaluate the effects of hysterectomy on rat ovaries and the possible protective role of peroxisome proliferator- activated receptor gamma (PPAR-γ) agonist-rosiglitazone against ovarian reserve decrement.
Forty-five adult Wistar albino rats were randomly divided into three groups. Hysterectomy was performed (n = 15) in group 1 [H]; 1 mg/kg/day PPAR-γ agonist/rosiglitazone was used for 50 days after hysterectomy (n = 15) in group 2 [H + R]; a sham operation was performed (n = 15) in group 3 [control, C]. Blood samples were collected for anti-Müllerian hormone (AMH) evaluation in all groups and simultaneous ovarian Doppler examination was performed in [H] and [H + R] groups before and after (50 days) hysterectomy. All animals were sacrificed to obtain ovaries for histological examination.
AMH levels were found to be significantly decreased at postoperative day 50 in all groups (P < 0.05). Histopathologic analysis showed that primary, preantral, and antral follicle counts were significantly higher in the [H] group as compared to the [C] and [H + R] groups (P < 0.05). There was no significant difference between the [C] and [H + R] groups in terms of follicle numbers (P > 0.05). In the ovarian Doppler blood flow analysis, all parameters were significantly decreased in group [H] (P < 0.05), but not in the [H + R] group (P > 0.05) on postoperative day 50.
Hysterectomy affects the histopathological structure of rat ovaries and PPAR-γ agonist-rosiglitazone improves the ovarian Doppler blood flow parameters.
背景/目的：评估子宫切除术对大鼠卵巢的影响，以及过氧化物酶体增殖物激活受体γ（PPAR-γ）激动剂-罗格列酮对卵巢储备减少的可能保护作用。
将 45 只成年 Wistar 白化大鼠随机分为三组。第 1 组（H）行子宫切除术（n = 15）；第 2 组（H + R）行子宫切除术，术后每天给予 1 mg/kg 的 PPAR-γ 激动剂/罗格列酮 50 天（n = 15）；第 3 组（C）行假手术（n = 15）。所有组均采集抗苗勒管激素（AMH）评估血样，同时在 [H] 和 [H + R] 组进行卵巢多普勒检查，术前和术后（50 天）。所有动物均行安乐死以获取卵巢进行组织学检查。
所有组术后第 50 天 AMH 水平均显著降低（P < 0.05）。组织病理学分析显示，[H]组初级、前腔卵泡和腔卵泡计数明显高于 [C]和 [H + R]组（P < 0.05）。[C]和 [H + R]组之间卵泡数量无显著差异（P > 0.05）。在卵巢多普勒血流分析中，[H]组所有参数均显著降低（P < 0.05），但 [H + R]组无显著变化（P > 0.05）。
子宫切除术影响大鼠卵巢的组织病理学结构，PPAR-γ 激动剂-罗格列酮可改善卵巢多普勒血流参数。
