Wang Zi, Huang Xin, Zan Tao, Li Qingfeng, Li Haizhou
Department of Plastic and Reconstructive Surgery, Ninth People's Hospital, Medical School of Shanghai Jiao Tong University, 639 Zhizaoju Road, Shanghai 200011, China.
Burns Trauma. 2020 May 6;8:tkaa013. doi: 10.1093/burnst/tkaa013. eCollection 2020.
Pathological scars might cause a distorted appearance and restricted mobility, and the study of scar pathophysiology has been hindered by the absence of a reliable model. In this study, we introduce a model with a modified device to induce controlled tension on a wound healing by secondary intention to overcome the shortcomings of the model generated by Aarabi . We investigated and recommend an induction of 0.1 N/mm tension on day 7 for 14 days to mimic the characteristics of human scars. A 3.5-fold increase in scar tissue and a 2-fold increase in collagen production were induced by the modified model. Histologically, the modified method increased scar thickness. However, no significant difference was found in cell density between the two groups. This modified procedure significantly increased scar tissue, which could be used for further cellular and biomolecular research. The mechanical force applied to the wound became measurable and controllable. This method is more convenient for researchers to observe in real-time and for providing timely adjustments of the tension used in this modified model.
病理性瘢痕可能会导致外观变形和活动受限，而瘢痕病理生理学的研究因缺乏可靠模型而受阻。在本研究中，我们引入了一种使用改良装置的模型，通过二期愈合对伤口愈合施加可控张力，以克服阿拉比所构建模型的缺点。我们研究并推荐在第7天施加0.1 N/mm的张力，持续14天，以模拟人类瘢痕的特征。改良模型使瘢痕组织增加了3.5倍，胶原蛋白生成增加了2倍。从组织学上看，改良方法增加了瘢痕厚度。然而，两组之间的细胞密度没有显著差异。这种改良程序显著增加了瘢痕组织，可用于进一步的细胞和生物分子研究。施加于伤口的机械力变得可测量和可控。这种方法更便于研究人员实时观察，并及时调整此改良模型中使用的张力。
