Hansen G S, Dupont B, Faber V, Jakobsen B K, Juhl F, Nielsen L S, Svejgaard A, Thomsen M, Wiik A
Scand J Immunol. 1977;6(4):299-303. doi: 10.1111/j.1365-3083.1977.tb00397.x.
Specific HLA antibodies were used to eliminate donor and recipient cells, respectively, from lymphocyte suspensions prepared from the blood of a child who had been transplanted with bone marrow from an HLA-A- and HLA-B-incompatible, HLA-D-compatible donor. About 70% of the lymphocytes were of donor HLA type, the remaining of recipient type. The phytohemagglutinin-responsive lymphocytes were exclusively limited to the lymphocyte population carrying donor-type HLA antigens. Membrane immunofluorescence investigations of the donor and recipient populations showed a low percentage of IgM-positive lymphocytes in the donor population and an extremely high proportion of IgM-positive lymphocytes in the recipient population. About 90% of the donor lymphocytes were T cells, as judged by their capacity to form rosettes between sheep erythrocytes and T lymphocytes; no cells in the recipient cell population expressed this ability.
使用特异性HLA抗体分别从一名接受了来自HLA - A和HLA - B不相容但HLA - D相容供体骨髓移植的儿童血液制备的淋巴细胞悬液中去除供体和受体细胞。约70%的淋巴细胞为供体HLA类型，其余为受体类型。对植物血凝素产生反应的淋巴细胞仅局限于携带供体型HLA抗原的淋巴细胞群体。对供体和受体群体进行的膜免疫荧光研究显示，供体群体中IgM阳性淋巴细胞的比例较低，而受体群体中IgM阳性淋巴细胞的比例极高。根据绵羊红细胞与T淋巴细胞形成花环的能力判断，约90%的供体淋巴细胞为T细胞；受体细胞群体中没有细胞表现出这种能力。
