Kofler Barbara, Laimer Johannes, Bruckmoser Emanuel, Steinbichler Teresa B, Runge Annette, Schartinger Volker H, von Laer Dorothee, Borena Wegene
Department of Otorhinolaryngology, Medical University of Innsbruck, Anichstrasse 35, 6020 Innsbruck, Austria.
University Hospital of Cranio-Maxillofacial and Oral Surgery, Medical University of Innsbruck, 6020 Innsbruck, Austria.
Cancers (Basel). 2020 May 8;12(5):1192. doi: 10.3390/cancers12051192.
: Certain high-risk (hr) types of human papillomavirus (HPV) can cause cervical cancer in women and penile cancer in men. Hr-HPV can also cause cancers of the oropharynx and anus in both sexes. In the anal and cervical region, a contribution of co-infections with Ureaplasma spp. on the persistence of the hr-HPV infection by a profound inflammatory state is suggested. Here, we investigated if non-HPV sexually transmitted infections are associated with oropharyngeal carcinoma (OPC). : In this case-control study, a brush test directly from the tumor surface of OPC patients (study group) and from the oropharynx of healthy volunteers (control group), both groups matching in age and sex, was performed. HPV subtypes were detected using a commercially available test kit. For non-HPV sexually transmitted infections (Ureaplasma spp., Chlamydia trachomatis, Mycoplasma hominis, and Mycoplasma genitalium), a multiplex nucleic acid amplification approach was performed. : In the study group, 96 patients (23 female/73 male), with histologically confirmed OPC and in the control group 112 patients (19 female/93 male), were included. Oropharyngeal hr-HPV-positivity was detected in 68% (65/96 patients) of the study group and 1.8% (2/112 patients) of the control group ( < 0.001). In three patients in the study group, Ureaplasma spp. was detected, whereas no patient was Ureaplasma spp. positive in the control group ( = 0.097). Chlamydia trachomatis, Mycoplasma hominis, and Mycoplasma genitalium were negative in both groups. : Based on the current study, the prevalence of oropharyngeal Ureaplasma spp. among patients with OPC is low and does not support a role in oropharyngeal cancer. However, the detection of the pathogen only among OPC patients but not in the healthy individuals might indicate a potential role and needs further elucidation.
某些高危（hr）型人乳头瘤病毒（HPV）可导致女性宫颈癌和男性阴茎癌。高危型HPV还可导致两性的口咽癌和肛门癌。在肛门和宫颈区域，有人提出解脲脲原体属的合并感染通过严重的炎症状态对高危型HPV感染的持续存在有影响。在此，我们研究了非HPV性传播感染是否与口咽癌（OPC）有关。
在这项病例对照研究中，对口咽癌患者（研究组）的肿瘤表面和健康志愿者（对照组）的口咽进行了直接刷检，两组在年龄和性别上相匹配。使用市售检测试剂盒检测HPV亚型。对于非HPV性传播感染（解脲脲原体属、沙眼衣原体、人型支原体和生殖支原体），采用多重核酸扩增方法。
研究组纳入了96例经组织学确诊为口咽癌的患者（23例女性/73例男性），对照组纳入了112例患者（19例女性/93例男性）。研究组68%（65/96例患者）检测到口咽高危型HPV阳性，对照组为1.8%（2/112例患者）（P<0.001）。研究组有3例患者检测到解脲脲原体属，而对照组无患者解脲脲原体属阳性（P = 0.097）。两组沙眼衣原体、人型支原体和生殖支原体均为阴性。
基于当前研究，口咽癌患者中口咽部解脲脲原体属的患病率较低，不支持其在口咽癌中的作用。然而，仅在口咽癌患者中检测到该病原体而在健康个体中未检测到，这可能表明其具有潜在作用，需要进一步阐明。
