Strydom D J
Center for Biochemical and Biophysical Sciences and Medicine, Harvard Medical School, Boston, Massachusetts 02115.
Anal Biochem. 1988 Nov 1;174(2):679-686. doi: 10.1016/0003-2697(88)90073-5.
Proteins and peptides are digested with anhydrous hydrazine and the free amino acids, released from their carboxyl termini, are analyzed by reverse-phase chromatography as phenylthiocarbamyl (PTC) derivatives. Most of the potentially interfering hydrazides are removed due to the insolubility of their derivatives in the solution used for dissolving the phenylthiocarbamyl-amino acids. This methodology can be used to analyze peptides and proteins in quantities smaller than 100 pmol. The method is here employed for the screening of carboxyl termini in peptide fractions originating from an HPLC chromatogram.
蛋白质和肽用无水肼消化，从其羧基末端释放的游离氨基酸作为苯硫代甲酰（PTC）衍生物通过反相色谱法进行分析。由于其衍生物在用于溶解苯硫代甲酰基氨基酸的溶液中不溶，大部分潜在干扰性酰肼被去除。该方法可用于分析小于100皮摩尔量的肽和蛋白质。本文采用该方法对源自高效液相色谱图的肽馏分中的羧基末端进行筛选。
