Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, China.
Academy of Agricultural Sciences, Southwest University, Chongqing, China.
Pest Manag Sci. 2020 Sep;76(9):2932-2943. doi: 10.1002/ps.5891. Epub 2020 Jun 13.
Bactrocera dorsalis (Hendel) is a notorious agricultural pest worldwide, and its resistance to insecticides is a major obstacle in successful control. Cytochrome P450s (P450s) are major metabolic enzymes associated with insecticide resistance. The genome of B. dorsalis was sequenced recently, allowing an integrated genome-wide analysis of P450 genes (P450s) and the analysis of correlations between these genes and insecticide resistance in this pest.
Totally, 101 P450s were identified in the B. dorsalis genome and classified into four clans, 25 families and 57 subfamilies. Quantitative reverse transcription polymerase chain reaction results showed that most of these genes were highly expressed in adults (46) and in metabolic tissues, including the fatbody (63), midgut (61) and Malphagian tubules (66). In a malathion-resistant strain, 13 and 9 genes were significantly upregulated and downregulated, respectively, compared with a susceptible strain, and these genes were screened as candidate genes associated with malathion resistance.
This study provides useful information for understanding the evolution and potential functions of P450s in B. dorsalis, and the results lay the foundation for further studies on the correlations between P450s and malathion resistance in B. dorsalis. © 2020 Society of Chemical Industry.
实蝇属的桔小实蝇（Hendel）是一种世界性的农业害虫，其对杀虫剂的抗性是成功防治的主要障碍。细胞色素 P450s（P450s）是与杀虫剂抗性相关的主要代谢酶。桔小实蝇的基因组最近被测序，允许对 P450 基因（P450s）进行全基因组综合分析，并分析这些基因与该害虫的杀虫剂抗性之间的相关性。
在桔小实蝇基因组中鉴定出 101 个 P450s，分为四个族、25 个家族和 57 个亚家族。定量逆转录聚合酶链反应结果表明，这些基因中的大多数在成虫（46）和代谢组织中高度表达，包括脂肪体（63）、中肠（61）和 Malphagian 小管（66）。在马拉硫磷抗性品系中，与敏感品系相比，分别有 13 个和 9 个基因显著上调和下调，这些基因被筛选为与马拉硫磷抗性相关的候选基因。
本研究为了解 P450s 在桔小实蝇中的进化和潜在功能提供了有用的信息，为进一步研究 P450s 与桔小实蝇马拉硫磷抗性之间的相关性奠定了基础。 © 2020 化学工业协会。
