Department of Plant Protection, College of Agriculture, Shiraz University, Shiraz 71441-65186, Iran.
Wageningen University and Research, Business Unit Biointeractions and Plant Health, 6700 AA, Wageningen, The Netherlands.
Phytopathology. 2020 Oct;110(10):1647-1656. doi: 10.1094/PHYTO-03-20-0072-R. Epub 2020 Aug 14.
Bacterial wilt and brown rot disease caused by species complex (RSSC) is one of the major constraints of potato () production around the globe. During 2017 to 2018, an extensive field survey was conducted in six potato-growing provinces of Iran to monitor the status of bacterial wilt disease. Pathogenicity and host range assays using 59 bacterial strains isolated in Iran showed that they were pathogenic on eggplant, red nightshade, pepper, potato and tomato, while nonpathogenic on common bean, cowpea, cucumber, sunflower, zinnia and zucchini. PCR-based diagnosis revealed that the strains belong to the phylotype IIB/sequevar 1 (IIB/I) lineage of the RSSC. Furthermore, a five-gene multilocus sequence analysis and typing (, , , , and ) confirmed the phylogenetically near-homogeneous nature of the strains within IIB/I lineage. Four sequence types were identified among 58 IIB/1 strains isolated in Iran. Phylogenetically near-homogeneous nature of the strains in Iran raise questions about the mode of inoculum entry of the bacterial wilt pathogen into the country (one-time introduction versus multiple introductions), while the geographic origin of the Iranian strains remains undetermined. Furthermore, sequence typing showed that there were shared alleles (haplotypes) and sequence types among the strains isolated in geographically distant areas in Iran, suggesting intranational transmission of the pathogen in the country.
由 种复合体（RSSC）引起的细菌性萎蔫病和褐腐病是全球马铃薯（）生产的主要制约因素之一。在 2017 年至 2018 年期间，在伊朗的六个马铃薯种植省份进行了广泛的田间调查，以监测细菌性萎蔫病的状况。使用在伊朗分离的 59 株细菌进行的致病性和宿主范围测定表明，它们对茄子、红茄、辣椒、马铃薯和番茄具有致病性，而对普通豆、豇豆、黄瓜、向日葵、百日草和南瓜则没有致病性。基于 PCR 的诊断表明，这些菌株属于 RSSC 的 phylotype IIB/sequevar 1 (IIB/I)谱系。此外，对 5 个基因多位点序列分析和分型（、、、、和）证实了 IIB/I 谱系内菌株的系统发育近同源性。在伊朗分离的 58 株 IIB/1 菌株中鉴定出 4 种序列型。伊朗菌株的系统发育近同源性引发了关于细菌性萎蔫病原菌进入该国的接种体进入模式（一次性引入与多次引入）的问题，而伊朗 菌株的地理起源仍未确定。此外，序列分型表明，在伊朗地理上遥远的地区分离的菌株之间存在共享等位基因（单倍型）和序列型，表明该病原体在该国的国内传播。
