State Key Laboratory of Crop Stress Biology for Arid Areas, and Key Laboratory of Integrated Pest Management on Crops in Northwestern Loess Plateau, Ministry of Agriculture, College of Plant Protection, Northwest A&F University, Yangling 712100, Shaanxi, China.
J Agric Food Chem. 2020 Jun 3;68(22):6076-6083. doi: 10.1021/acs.jafc.0c01486. Epub 2020 May 19.
Carboxylesterases (CarEs) are a multigene superfamily of metabolic enzymes involved in metabolic detoxification of xenobiotics. In this study, an αesterase gene () was identified from . Phylogenetic analysis classified into the esterase clade. Developmental expression analysis indicated that was significantly expressed in the second to fourth larval stages. Tissue-specific expression analysis indicated that was highly expressed in the larval midgut. After exposure to LC of malathion, the CarE activity of was induced and the transcriptional level of was significantly up-regulated. Silencing of significantly increased the susceptibility of larvae to malathion. Inhibition assays indicated that malathion significantly inhibited BoαE1 activity. GC-MS assay showed that BoαE1 possesses hydrolase activity toward malathion and participates in the detoxification of malathion. These results strongly suggest that plays a crucial role in detoxification of malathion in .
羧酸酯酶（CarEs）是参与代谢解毒的代谢酶的一个多基因超家族。在本研究中，从 中鉴定出一个α酯酶基因 ()。系统发育分析将 归类为酯酶分支。发育表达分析表明 ，在第二至第四龄幼虫期显著表达。组织特异性表达分析表明 ，在幼虫中肠中高度表达。暴露于乐果的 LC 后， 的 CarE 活性被诱导， 的转录水平显著上调。 沉默显著增加了 幼虫对乐果的敏感性。抑制试验 表明乐果显著抑制 BoαE1 的活性。GC-MS 分析表明 BoαE1 对乐果具有水解酶活性，参与乐果的解毒。这些结果强烈表明 ，在 对乐果的解毒中起着至关重要的作用。
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