Yao Ping-Hong, Mobarak Syed Husne, Yang Mao-Fa, Hu Chao-Xing
Provincial Key Laboratory for Agricultural Pest Management of Mountainous Region, Institute of Entomology, Guizhou University, Guiyang, 550025, China.
BMC Genomics. 2025 Jan 6;26(1):14. doi: 10.1186/s12864-024-11185-2.
The fall armyworm (FAW) Spodoptera frugiperda, a highly invasive, polyphagous pest, poses a global agricultural threat. It has two strains, the C-corn and R-rice strains, each with distinct host preferences. This study compares detoxification enzyme gene families across these strains and related Spodoptera species to explore their adaptation to diverse host plant metabolites.
A total of 1,995 detoxification-related genes, including cytochrome P450 monooxygenases (CYPs), carboxylesterases (COEs), glutathione S-transferases (GSTs), UDP-glucuronosyltransferases (UGTs), and ATP-binding cassette transporters (ABCs), were identified across the genus Spodoptera, including S. littoralis, S. litura, S. picta, S. exigua, and both FAW strains. A higher abundance of phase I detoxification enzymes (CYPs and COEs) and GSTs was observed in Spodoptera species, while FAW strains exhibited fewer detoxification genes, with notable differences in copy numbers between the C and R strains. Analyses at the subfamily level revealed significant variation in gene distribution and expression, particularly within phase I and II detoxification enzymes. Expansions in CYP6AE were detected in the C strain, while contractions in GST-ε, CYP9A, CYP4M, UGT33B, and UGT33F occurred in both strains. In contrast, no substantial variation was observed in phase III ABC enzymes. Functional predictions and protein interaction networks suggest a broader expansion of metabolism-related genes in the R strain compared to the C strain.
These findings emphasize the pivotal role of phase I and II detoxification enzymes in host adaptation, providing molecular insights into FAW's capacity for host range expansion, which are crucial for devising targeted and sustainable pest management strategies.
草地贪夜蛾（FAW），即草地贪夜蛾，是一种极具入侵性的多食性害虫，对全球农业构成威胁。它有两个品系，即C玉米品系和R水稻品系，每个品系都有不同的寄主偏好。本研究比较了这些品系以及相关夜蛾科物种的解毒酶基因家族，以探索它们对不同寄主植物代谢物的适应性。
在夜蛾属中，包括滨海夜蛾、斜纹夜蛾、彩纹夜蛾、甜菜夜蛾以及草地贪夜蛾的两个品系，共鉴定出1995个与解毒相关的基因，包括细胞色素P450单加氧酶（CYPs）、羧酸酯酶（COEs）、谷胱甘肽S-转移酶（GSTs）、UDP-葡萄糖醛酸基转移酶（UGTs）和ATP结合盒转运蛋白（ABCs）。在夜蛾科物种中观察到I相解毒酶（CYPs和COEs）和GSTs的丰度较高，而草地贪夜蛾品系的解毒基因较少，C品系和R品系之间的拷贝数存在显著差异。亚家族水平的分析揭示了基因分布和表达的显著差异，特别是在I相和II相解毒酶中。在C品系中检测到CYP6AE的扩增，而在两个品系中GST-ε、CYP9A、CYP4M、UGT33B和UGT33F均出现收缩。相比之下，III相ABC酶未观察到实质性变化。功能预测和蛋白质相互作用网络表明，与C品系相比，R品系中与代谢相关的基因有更广泛的扩增。
这些发现强调了I相和II相解毒酶在寄主适应中的关键作用，为草地贪夜蛾寄主范围扩展能力提供了分子见解，这对于制定有针对性的可持续害虫管理策略至关重要。
