血清和血浆中存在一种依赖腺嘌呤的抑制剂，它会延缓原代培养的晶状体上皮细胞的再激活。
Evidence for an adenine-dependent inhibitor in serum and plasma which retards the reactivation of lens epithelial cells in primary culture.
作者信息
Glaesser D, Ngoli D, Iwig M
机构信息
Institute of Biochemistry, Martin Luther University, GDR, Halle, Saale.
出版信息
Biomed Biochim Acta. 1988;47(4-5):373-82.
Primary culture of epithelium-capsule-preparations (ECP) from eye lenses can be performed in a HEPES- and EDTA-buffered salt solution. Addition of serum causes an initial retraction of the epithelium, and thereafter it stimulates spreading and flattening of the cells. In the presence of 10 microM adenine the serum-evoked retraction is strengthened, and the serum-stimulated spreading is inhibited. These effects of adenine are mediated by a low molecular weight serum factor, which was partially purified.
眼晶状体上皮-囊膜制剂（ECP）的原代培养可在含有羟乙基哌嗪乙磺酸（HEPES）和乙二胺四乙酸（EDTA）的缓冲盐溶液中进行。添加血清会导致上皮细胞最初发生收缩，随后刺激细胞铺展并变平。在存在10微摩尔腺嘌呤的情况下，血清引起的收缩会增强，而血清刺激的铺展则受到抑制。腺嘌呤的这些作用是由一种低分子量血清因子介导的，该因子已被部分纯化。
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