Lee Jaesung, Pascall Melvin A
Department of Food Science and Technology Ohio State University Columbus OH USA.
Food Sci Nutr. 2020 Mar 24;8(5):2472-2477. doi: 10.1002/fsn3.1537. eCollection 2020 May.
Using polymerization and immobilization techniques, the loss of antimicrobial efficacy of a quaternary ammonium compound (QAC) was minimized by decreasing its solubility and crosslinking it to metal substrates. The survivability of and K12 inoculated to silane QAC coated metal surfaces was compared with uncoated metal surfaces at different treatment conditions for up to 6 months storage. Resilience of the coating material to repeated cleaning, up to 20 washing and rinsing cycles, was also investigated. No evidence of bacteria viability (>5 log reduction of colony-forming unit) was observed for when they were inoculated onto coated surfaces stored for 3 months, whereas was reduced by 3 to 4-logs. For the viable cells on the coated surfaces, >5 log reductions were achieved even after the coated surfaces were cleaned by 20 washing and rinsing cycles prior to the cells' inoculation. For the cells, ~ 2 log reductions were achieved after 5 cleaning cycles and <1 log reduction after 10 or more cleaning procedures. Overall, the results showed that the coating had antimicrobial activity against Gram-positive bacteria while it showed moderate activity to Gram-negative bacteria.
采用聚合和固定化技术，通过降低季铵化合物（QAC）的溶解度并将其交联到金属基底上，使QAC抗菌效能的损失降至最低。在不同处理条件下，将 和K12接种到硅烷QAC涂层金属表面，并与未涂层的金属表面进行比较，储存长达6个月。还研究了涂层材料对重复清洗（多达20次洗涤和漂洗循环）的耐受性。当将 接种到储存3个月的涂层表面时，未观察到细菌存活的迹象（菌落形成单位减少>5个对数），而 减少了3至4个对数。对于涂层表面上存活的 细胞，即使在接种细胞之前对涂层表面进行20次洗涤和漂洗循环的清洁后，仍实现了>5个对数的减少。对于 细胞，在5次清洁循环后减少了约2个对数，在10次或更多清洁程序后减少<1个对数。总体而言，结果表明该涂层对革兰氏阳性菌具有抗菌活性，而对革兰氏阴性菌具有中等活性。
