Dumon M F, Dang Q Q, Salvayre R, Clerc M, Douste-Blazy L
Laboratoire de Biochimie Médicale A, Université de Bordeaux II, France.
Chem Phys Lipids. 1988 Dec;49(3):153-60. doi: 10.1016/0009-3084(88)90002-3.
Fluorescence quenching by iodide ions has been found to be higher in isolated Tangier low density lipoprotein (LDL2) than in isolated normal LDL2. Apolipoprotein (apo) B-100 is the main protein component of these lipoproteins and its tryptophanyl residues (Trp) are known to be the most hydrophobic and to be responsible for protein fluorescence. Trp exposure can thus be calculated; it was 0.50 in Tangier and 0.42 and 0.41 in insulin-dependent diabetics (IDD) and normal controls, respectively. The greater fluorescence quenching of Tangier LDL2 reveals a shallower embedding of Trp which is principally due to a lowered free cholesterol (FC) level in the shell and a smaller lipid core, itself dependent on a drop in cholesterol esters (CE). This is in accordance with the electrophoretic properties of Tangier LDL2 and suggests that Tangier LDL2 may be considered to be modified.
已发现，碘离子对分离出的丹吉尔低密度脂蛋白（LDL2）的荧光猝灭作用比对分离出的正常LDL2的荧光猝灭作用更强。载脂蛋白（apo）B-100是这些脂蛋白的主要蛋白质成分，已知其色氨酸残基（Trp）是疏水性最强的，并且是蛋白质荧光的来源。因此，可以计算色氨酸的暴露程度；在丹吉尔病患者中为0.50，在胰岛素依赖型糖尿病患者（IDD）和正常对照中分别为0.42和0.41。丹吉尔LDL2更强的荧光猝灭表明色氨酸的嵌入较浅，这主要是由于外壳中游离胆固醇（FC）水平降低以及脂质核心较小，而脂质核心本身又取决于胆固醇酯（CE）的减少。这与丹吉尔LDL2的电泳特性相符，并表明丹吉尔LDL2可能被认为是经过修饰的。
