Maize Research Institute, Shandong Academy of Agricultural Sciences/National Engineering Laboratory of Wheat and Maize/Key Laboratory of Biology and Genetic Improvement of Maize in Northern Yellow-Huai River Plain, Ministry of Agriculture and Rural Affairs, Jinan, China.
Institute of Plant Protection, Shandong Academy of Agricultural Sciences, Jinan, China.
PLoS One. 2020 May 14;15(5):e0232812. doi: 10.1371/journal.pone.0232812. eCollection 2020.
Sulfoxaflor, the first commercially available sulfoximine insecticide, has been used for the control of sap-feeding insect pests such as plant bugs and aphids on a variety of crops. However, its sublethal effects on the mirid bug Apolygus lucorum, one of the key insect pests of Bt cotton and fruit trees in China, have not been fully examined. Here, we evaluated the demography and feeding behaviour of A. lucorum exposed to sulfoxaflor. The leaf-dipping bioassay showed that the LC10 and LC30 of sulfoxaflor against 3rd-instar nymphs of this insect were 1.23 and 8.37 mg L-1, respectively. The LC10 significantly extended the nymphal duration and decreased the oviposition period by 5.29 days and female fecundity by 56.99% in the parent generation (F0). The longer duration of egg, 5th-instar nymphs, preadult, and male adult longevity were observed in the F1 generation (F1) at LC10. At the LC30, the duration of egg and 1st-instar nymph, female adult longevity, and oviposition period of the F1 were significantly shorter, while the nymphal duration in the F0 and duration of 5th-instar nymphs, preadult survival rate, and male adult longevity in the F1 significantly increased. The net reproductive rate (R0), intrinsic rate of increase (r), and finite rate of increase (λ) in the F1 were not significantly affected by these two concentrations, whereas the mean generation time (T) was lower at the LC30. Additionally, the probe counts and cells mixture feeding time were markedly lengthened by the LC10 and LC30, respectively, when A. lucorum nymphs exposed to sulfoxaflor fed on Bt cotton plants without insecticides. These results clearly indicate that sulfoxaflor causes sublethal effects on A. lucorum and the transgenerational effects depend on the tested concentrations.
砜虫酰胺是第一种商业上可获得的亚砜亚胺类杀虫剂，已被用于防治多种作物上的刺吸式昆虫害虫，如盲蝽和蚜虫。然而，其对中国棉花和果树的关键害虫之一——棉盲蝽 Apolygus lucorum 的亚致死效应尚未得到充分研究。在这里，我们评估了接触砜虫酰胺对 A. lucorum 的种群动态和取食行为的影响。叶浸生物测定表明，该药剂对 3 龄若虫的 LC10 和 LC30 分别为 1.23 和 8.37mg/L。LC10 显著延长了若虫期，并使雌成虫的产卵期缩短了 5.29 天，使雌成虫的产卵量减少了 56.99%（亲代 F0）。在 LC10 下，F1 代观察到卵期、5 龄若虫期、预蛹期和雄成虫寿命延长。在 LC30 下，F1 代的卵期和 1 龄若虫期、雌成虫寿命和产卵期明显缩短，而 F0 代的若虫期和 5 龄若虫期、预蛹存活率和雄成虫寿命明显延长。F1 代的净生殖率（R0）、内禀增长率（r）和周限增长率（λ）在这两个浓度下均无显著影响，而 LC30 时的平均世代时间（T）较低。此外，当接触砜虫酰胺的棉盲蝽取食不含杀虫剂的转 Bt 棉植株时，LC10 和 LC30 分别显著延长了探针计数和细胞混合物取食时间。这些结果清楚地表明，砜虫酰胺对棉盲蝽造成亚致死效应，并且这种跨代效应取决于测试浓度。
